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. 2019 Jun;16(6):497-500.
doi: 10.1038/s41592-019-0393-z. Epub 2019 Apr 29.

Instantaneous isotropic volumetric imaging of fast biological processes

Nils Wagner #  1 , Nils Norlin #  1   2 , Jakob Gierten  3   4 , Gustavo de Medeiros  1   5 , Bálint Balázs  1 , Joachim Wittbrodt  3 , Lars Hufnagel  6 , Robert Prevedel  7   8   9
Affiliations

Instantaneous isotropic volumetric imaging of fast biological processes

Nils Wagner et al. Nat Methods. 2019 Jun.

Abstract

To capture highly dynamic biological processes at cellular resolution is a recurring challenge in biology. Here we show that combining selective-volume illumination with simultaneous acquisition of orthogonal light fields yields three-dimensional images with high, isotropic spatial resolution and a significant reduction of reconstruction artefacts, thereby overcoming current limitations of light-field microscopy implementations. We demonstrate medaka heart and blood flow imaging at single-cell resolution and free of motion artefacts at volume rates of up to 200 Hz.

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