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. 2019 Jun;8(6):654-660.
doi: 10.1530/EC-19-0012.

GC/MS-based metabolomics strategy to analyze the effect of exercise intervention in diabetic rats

Li Jing  1 , Wang Chengji  1
Affiliations

GC/MS-based metabolomics strategy to analyze the effect of exercise intervention in diabetic rats

Li Jing et al. Endocr Connect. 2019 Jun.

Abstract

Metabolomics was used to explore the effect of exercise intervention on type 2 diabetes. The rat model of type 2 diabetes was induced by an injection of streptozocin (30 mg/kg), after fed with 8-week high-fat diet. The rats were divided into three groups: the control group, the diabetic model group (DM) and the diabetes + exercise group (DME). After exercise for 10 weeks, blood samples were collected to test biomedical indexes, and 24-h urine samples were collected for the metabolomics experiment. In the DME group, fasting blood glucose (FBG), both total cholesterol (TC) and total plasma triglycerides (TG), were decreased significantly, compared with those in the DM group. Based on gas chromatography-mass spectrometry (GC/MS), a urinary metabolomics method was used to study the mechanism of exercise intervention on diabetes mellitus. Based on the principal component analysis (PCA), it was found that the DM group and control group were separated into two different clusters. The DME group was located between the DM group and the control group, closer to the control group. Twelve significantly changed metabolites of diabetes mellitus were detected and identified, including glycolate, 4-methyl phenol, benzoic acid, 1H-indole, arabinitol, threitol, ribonic acid, malic acid, 2,3-dihydroxy-butanoic, aminomalonic acid, l-ascorbic acid and 3-hydroxy hexanedioic acid. After exercise, seven metabolites were significantly changed, compared with the control group, the relative contents of benzoic acid, aminomalonic acid, tetrabutyl alcohol and ribonucleic acid in the diabetic exercise group decreased significantly. The relative contents of 2,3-dihydroxybutyric acid, l-ascorbic acid and 3-hydroxy adipic acid increased significantly. l-ascorbic acid and aminomalonic acid which related with the oxidative stress were significantly regulated to normal. The results showed that exercise could display anti-hyperglycemic and anti-hyperlipidemic effects. The exercise had antioxidation function in preventing the occurrence of complications with diabetes mellitus to some extent. The work illustrates that the metabolomics method is a useful tool to study the mechanism of exercise treatment.

Keywords: exercise; gas chromatography-mass spectrometry; metabolomics; type 2 diabetes.

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Figures

Figure 1
Figure 1
Oral glucose tolerance test (OGTT) curves of rats in different groups. ※P < 0.05, compared with the control group; △P < 0.05, compared with the model group.
Figure 2
Figure 2
Typical total ion current (TIC) chromatogram of a urine sample obtained from the diabetic rat.
Figure 3
Figure 3
PCA results of rat urine samples from different groups.
Figure 4
Figure 4
Peak area ratios of potential biomarkers to internal standard in different groups. *P < 0.05, compared with the model group.
See this image and copyright information in PMC

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References

    1. Guariguata L, Whiting DR, Hambleton I, Beagley J, Linnenkamp U, Shaw JE. Global estimates of diabetes prevalence for 2013 and projections for 2035. Diabetes Research and Clinical Practice 2014. 103 137–149. (10.1016/j.diabres.2013.11.002) - DOI - PubMed
    1. Long AN, Dagogo-Jack S. Comorbidities of diabetes and hypertension: mechanisms and approach to target organ protection. Journal of Clinical Hypertension 2011. 13 244–251. (10.1111/j.1751-7176.2011.00434.x) - DOI - PMC - PubMed
    1. DiPietro L, Gribok A, Stevens MS, Hamm LF, Rumpler W. Three 15-min bouts of moderate postmeal walking significantly improves 24-h glycemic control in older people at risk for impaired glucose tolerance. Diabetes Care 2013. 36 3262–3268. (10.2337/dc13-0084) - DOI - PMC - PubMed
    1. Qiu S, Cai X, Yin H, Sun Z, Zügel M, Steinacker JM, Schumann U. Exercise training and endothelial function in patients with type 2 diabetes: a meta-analysis. Cardiovascular Diabetology 2018. 17 64 (10.1186/s12933-018-0711-2) - DOI - PMC - PubMed
    1. Sallam N, Laher I. Exercise modulates oxidative stress and inflammation in aging and cardiovascular diseases. Oxidative Medicine and Cellular Longevity 2016. 2016 7239639 (10.1155/2016/7239639) - DOI - PMC - PubMed