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. 1987 Apr;169(4):1651-5.
doi: 10.1128/jb.169.4.1651-1655.1987.

Purification and characterization of ribitol-5-phosphate and xylitol-5-phosphate dehydrogenases from strains of Lactobacillus casei

S Z Hausman, J London

Purification and characterization of ribitol-5-phosphate and xylitol-5-phosphate dehydrogenases from strains of Lactobacillus casei

S Z Hausman et al. J Bacteriol. 1987 Apr.

Abstract

A simple three-step procedure is described which yields electrophoretically homogeneous preparations of ribitol-5-phosphate dehydrogenase and xylitol-5-phosphate dehydrogenase. The former enzyme is a 115,000-molecular-weight protein composed of two subunits of identical size and is specific for its substrate, ribitol. The xylitol-5-phosphate dehydrogenase exists as a tetrameric protein with a molecular weight of 180,000; this enzyme oxidizes the phosphate esters of both xylitol and D-arabitol. Characterization of the physical, kinetic, and immunological properties of the two enzymes suggests that the functionally similar enzymes may not be structurally related.

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References

    1. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4296-300 - PubMed
    1. J Infect Dis. 1962 May-Jun;110:258-67 - PubMed
    1. Ann N Y Acad Sci. 1964 Dec 28;121:404-27 - PubMed
    1. J Bacteriol. 1982 May;150(2):657-61 - PubMed
    1. J Bacteriol. 1979 Dec;140(3):949-54 - PubMed

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