Streptococcus periodonticum sp. nov., Isolated from Human Subgingival Dental Plaque of Periodontitis Lesion

Abstract

A novel facultative anaerobic and Gram-stain-positive coccus, designated strain ChDC F135^T, was isolated from human subgingival dental plaque of periodontitis lesion and was characterized by polyphasic taxonomic analysis. The 16S rRNA gene (16S rDNA) sequence of strain ChDC F135^T was closest to that of Streptococcus sinensis HKU4^T (98.2%), followed by Streptococcus intermedia SK54^T (97.0%), Streptococcus constellatus NCTC11325^T (96.0%), and Streptococcus anginosus NCTC 10713^T (95.7%). In contrast, phylogenetic analysis based on the superoxide dismutase gene (sodA) and the RNA polymerase beta-subunit gene (rpoB) showed that the nucleotide sequence similarities of strain ChDC F135^T were highly similar to the corresponding genes of S. anginosus NCTC 10713^T (99.2% and 97.6%, respectively), S. constellatus NCTC11325^T (87.8% and 91.4%, respectively), and S. intermedia SK54^T (85.8% and 91.2%, respectively) rather than those of S. sinensis HKU4^T (80.5% and 82.6%). The complete genome of strain ChDC F135^T consisted of 1,901,251 bp and the G+C content was 38.9 mol %. Average nucleotide identity value between strain ChDC F135^T and S. sinensis HKU4^T or S. anginosus NCTC 10713^T were 75.7% and 95.6%, respectively. The C_14:0 composition of the cellular fatty acids of strain ChDC F135^T (32.8%) was different from that of S. intermedia (6-8%), S. constellatus (6-13%), and S. anginosus (13-20%). Based on the results of phylogenetic and phenotypic analysis, strain ChDC F135^T (= KCOM 2412^T = JCM 33300^T) was classified as a type strain of a novel species of the genus Streptococcus, for which we proposed the name Streptococcus periodonticum sp. nov.