Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 May 4;30(3).
doi: 10.1515/jbcpp-2017-0176.

Antioxidant activity of crude ethanolic extract and fractions of Ziziphus mauritiana Lam. (Rhamnaceae) leaves from Burkina Faso

Estelle N H Youl  1 , Cyrille A P Ouédraogo  2 , Moustapha Gambo  2 , Moussa Ouédraogo  2 , Martin Kiendrebéogo  3 , Aristide Traoré  4 , Innocent Pierre Guissou  2
Affiliations

Antioxidant activity of crude ethanolic extract and fractions of Ziziphus mauritiana Lam. (Rhamnaceae) leaves from Burkina Faso

Estelle N H Youl et al. J Basic Clin Physiol Pharmacol. .

Abstract

Background Ziziphus mauritiana Lam. is a plant used in traditional medicine in Burkina Faso in the treatment of several diseases, of which diabetes is characterized by oxidative stress. The aim of this study was to evaluate the in vitro antioxidant potential of the extracts of leaves of this plant. Methods The crude hydroethanolic extract (HEE) of the leaves of Z. mauritiana and their partitionates in n-hexane, dichloromethane, and ethyl acetate, and in the residual aqueous solution (the F1, F2, F3, and F4 fractions, respectively) were first prepared. The content of polyphenols was determined and the antioxidant effects of the extracts were evaluated by their 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, inhibition of lipid peroxidation (TBARS), and the ferric reducing antioxidant power (FRAP). Results The HEE as well as the F3 and F4 fractions were rich in polyphenols with contents between 58 and 84 mg equivalent gallic acid per 100 mg. The flavonoid content was 4 mg quercetin equivalents in the HEE and the F4 fraction. Except for the F1 fraction, the HEE and the other fractions showed significant DPPH scavenging activity (IC50 between 8 and 12 μg/mL). The IC50 of TBARS by different extracts was in the range 1-5 μg/mL, and the FRAP activity was 7-85 mg ascorbic acid equivalent per 100 mg. Total polyphenol content was highly correlated with the antioxidant activities. Conclusions The HEE, F3, and F4 fractions were found to be the richest in polyphenols and had the best antioxidant activity. The antioxidant activity of the extracts of the leaves of Z. mauritiana is due to these polyphenolic compounds.

Keywords: Ziziphus mauritiana; antioxidant activity; polyphenol content.

PubMed Disclaimer

References

    1. Pincemail J, Bonjean K, Cayeux K, Defraigne JO. Mécanismes physiologiques de la défense antioxydante. Nutr Clin Metab 2002;16:233–9.
    1. Newsholme P, Rebelato E, Abdulkader F, Krause M, Carpinelli A, Curi R. Reactive oxygen and nitrogen species generation, antioxidant defenses, and β-cell function: a critical role for amino acids. J Endocrinol 2012;214:11–20.
    1. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007;39:44–84.
    1. Ahmad W, Ijaz B, Shabbiri K, Ahmed F, Rehman S. Oxidative toxicity in diabetes and Alzheimer’s disease: mechanisms behind ROS/RNS generation. J Biomed Sci 2017;24:76.
    1. Evans JL, Goldfine ID, Maddux BA, Grodsky GM. Are oxidative stress-activated signaling pathways mediators of insulin resistance and beta-cell dysfunction? Diabetes 2003;52:1–8.

LinkOut - more resources