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. 2019 Apr-Jun;20(2):70-75.

Expression Analysis of the CRISP2, CATSPER1, PATE1 and SEMG1 in the Sperm of Men with Idiopathic Asthenozoospermia

Zohreh Heidary  1 , Majid Zaki-Dizaji  1 , Kioomars Saliminejad  1 , Hamid Reza Khorramkhorshid  1   2
Affiliations

Expression Analysis of the CRISP2, CATSPER1, PATE1 and SEMG1 in the Sperm of Men with Idiopathic Asthenozoospermia

Zohreh Heidary et al. J Reprod Infertil. 2019 Apr-Jun.

Abstract

Background: The purpose of this study was to analyze the expression level of CRISP2, CATSPER1, PATE1 and SEMG1 genes in the sperm of men with asthenozoospermia (AZS). AZS is a cause of infertility in men in which the motility of the sperm is reduced. So far, a few genes have been associated with AZS; however, in most of the cases, its molecular etiology is unclear.

Methods: A total of 35 subjects with idiopathic AZS and 35 fertile and healthy men as control were included. In study after total RNA extraction and cDNA synthesis, relative quantification was performed. B2M was used as the normalizer gene and fold change was calculated by 2-ΔΔCt method. Mann-Whitney test was used to compare the expression levels between the case and control groups with significance level of p<0.05.

Results: Our results showed that CRISP2 (p=0.03) and SEMG1 (p=0.03) were significantly down-and up-regulated in AZS men respectively compared to the controls. But CATSPER1 and PATE1 did not show significant changes.

Conclusion: Down-regulation of CRISP2 and up-regulation of SEMG1 were associated with AZS, which could be suggested as the potential candidate genes for the development of a diagnostic marker or potentially for more studies for treatment of AZS.

Keywords: Asthenozoospermia; CATSPER1; CRISP2; Gene expression; Male infertility; PATE1; Real-time PCR; SEMG1; Sperm motility.

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Conflict of interest statement

Conflict of Interest The study was supported by the Avicenna Research Institute, Tehran, Iran.

Figures

Figure 1.
Figure 1.
ROC curve analysis of expression data. ROC curve comparing sensitivity and specificity of expression data for detection of AZS patients. ROC curve analyses suggest that expression of SEMG1 and CRISP2 have poor diagnostic value for AZS detection
See this image and copyright information in PMC

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