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. 2019 May 6;9(1):6985.
doi: 10.1038/s41598-019-43439-5.

Stability Mechanism of Two Soybean Protein-Phosphatidylcholine Nanoemulsion Preparation Methods from a Structural Perspective: A Raman Spectroscopy Analysis

Ying Zhu  1 , Yang Li  1   2 , Changling Wu  1 , Fei Teng  1 , Baokun Qi  1 , Xiaonan Zhang  1 , Linyi Zhou  1 , Guoping Yu  1 , Huan Wang  1 , Shuang Zhang  1 , Zhongjiang Wang  3 , Lianzhou Jiang  4   5
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Stability Mechanism of Two Soybean Protein-Phosphatidylcholine Nanoemulsion Preparation Methods from a Structural Perspective: A Raman Spectroscopy Analysis

Ying Zhu et al. Sci Rep. .

Abstract

Ultrasound treatment and high-pressure homogenization were used to prepare soybean protein (SP)-phosphatidylcholine (PC) nanoemulsions in this study. Nanoemulsions prepared by high-pressure homogenization were more stable. The structural changes of SP and PC under ultrasound treatment and high-pressure homogenization treatment were investigated by Raman spectroscopy. It could be concluded that ultrasound and high-pressure homogenization treatments increased both the content of α-helix and unordered structure but decreased that of β-structures of SP, while the interaction between SP and PC decreased α-helix content and also reduced unordered structure and β-sheet structure. Ultrasound treatment and high-pressure homogenization exposed more tryptophan and tyrosine residues to promote hydrophobic interaction between SP and PC, which was beneficial for stabilizing the nanoemulsion. The SP-PC interaction exerted a more significant effect on side chain structure than those observed under ultrasound treatment and high-pressure homogenization. The dominant g-g-t vibrational mode of the disulfide bond of soybean protein was not appreciably changed by the two preparations. High-pressure homogenization increased the disorder of lipid chains of PC, promoting SP-PC interaction and thereby increasing the stability of the nanoemulsion. The structural change provided a theoretical basis for preparation of two nanoemulsions.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Particle size distribution of the nanoemulsions prepared by ultrasound and high-pressure homogenization treatments.
Figure 2
Figure 2
Different microscopy of nanoemulsions under different preparation methods 3D Confocal laser scanning microscopy: (a) the nanoemulsion prepared by ultrasound; (b) the nanoemulsion prepared by high-pressure homogenization; Optical microscopy: (c) the nanoemulsion prepared by ultrasound; (d) the nanoemulsion prepared by high-pressure homogenization.
Figure 3
Figure 3
Raman spectra of nanoemulsions after different treatments: (a) the SP under ultrasound and high pressure homogenization; (b) the SP-PC nanoemulsions under ultrasound and high-pressure homogenization.
See this image and copyright information in PMC

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