Development and Fabrication of Vapor Cross-Linked Hyaluronan-Polyethylene Interpenetrating Polymer Network as a Biomaterial

Abstract

Flexible heart valve leaflets made from hyaluronan-enhanced linear low-density polyethylene interpenetrating polymeric network (HA-LLDPE IPN) films have been shown to provide good hemodynamics, but the resulting surfaces were not consistent; therefore, the present work tries to mitigate this problem by developing a vapor cross-linked HA-LLDPE IPN. Herein, the HA-LLDPE fabrication process is studied, and its parameters are varied to assess their effects on the IPN formation. Thermal analysis and gas chromatography-mass spectrometry were used to quantify the effects of different treatment conditions on material properties. Water contact angle goniometry, infrared spectroscopy, and toluidine blue O (TBO) staining were used to characterize the surface of the HA-LLDPE IPN. The results show that a hydrophilic surface is formed on HA-LLDPE, which is indicative of HA. HA surface density data from TBO staining show consistent HA distribution on the surface. The IPN fabrication process does not affect the tensile properties that make LLDPE an attractive material for use in flexible heart valve leaflets. The 28 day in vitro biological assays show HA-LLDPE to be noncytotoxic and resistant to enzymatic degradation. The HA-LLDPE showed less platelet adhesion and caused less platelet activation than the plain LLDPE or tissue culture polystyrene. All of the results indicate that vapor cross-linked HA-LLDPE IPN is a promising material for use as flexible leaflets for heart valve replacements.

Keywords: IPN; biomaterial; heart valve leaflets; hyaluronan; polyethylene; vapor cross-linking.