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. 2019 Sep;99(5):988-1002.
doi: 10.1111/tpj.14378. Epub 2019 Jun 7.

The ethylene response factor VaERF092 from Amur grape regulates the transcription factor VaWRKY33, improving cold tolerance

Xiaoming Sun  1   2 , Langlang Zhang  1 , Darren C J Wong  3 , Yi Wang  2 , Zhenfei Zhu  1 , Guangzhao Xu  2 , Qingfeng Wang  1 , Shaohua Li  2 , Zhenchang Liang  2 , Haiping Xin  1
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Free article

The ethylene response factor VaERF092 from Amur grape regulates the transcription factor VaWRKY33, improving cold tolerance

Xiaoming Sun et al. Plant J. 2019 Sep.
Free article

Abstract

Cold stress is a major limiting factor in grape (Vitis) productivity. In this study, we characterized a cold-responsive ethylene response factor (ERF) transcription factor, VaERF092, from Amur grape (Vitis amurensis). VaERF092 expression was induced by both low temperatures and the ethylene precursor 1-aminocyclopropane-1-carboxylate (ACC), but was suppressed by treatment with the ethylene inhibitor aminoethoxyvinylglycine (AVG) under cold conditions. Ectopic expression of VaERF092 in Arabidopsis thaliana enhanced cold tolerance. Co-expression network analysis of V. vinifera genes indicated that WRKY33 might be a downstream target of VaERF092. This hypothesis was supported by the fact that VaWRKY33 was expressed temporally after VaERF092 expression and could also be induced by cold and ACC, and inhibited by AVG. Yeast one-hybrid, transient β-glucuronidase (GUS) and dual-luciferase reporter assays provided evidence for an interaction between VaERF092 and a GCC-box element in the VaWRKY33 promoter. In addition, heterologous overexpression of VaWRKY33 in A. thaliana resulted in enhanced cold tolerance. VaERF092- and VaWRKY33 overexpressing grape calli showed lower low-temperature exothermic values than the empty vector (EV) calli, indicating enhanced tolerance to cold. Together, these results indicated that VaERF092 regulates VaWRKY33 through binding to its promoter GCC-box, leading to enhanced cold stress tolerance.

Keywords: Vitis amurensis; ERF transcription factor; GCC-box; WRKY transcription factor; cold stress.

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References

    1. Allen, M.D., Yamasaki, K., Ohme-Takagi, M., Tateno, M. and Suzuki, M. (1998) A novel mode of DNA recognition by a β-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA. EMBO J. 17, 5484-5496.
    1. Aubry, S., Kelly, S., Kumpers, B.M., Smith-Unna, R.D. and Hibberd, J.M. (2014) Deep evolutionary comparison of gene expression identifies parallel recruitment of trans-factors in two independent origins of C4 photosynthesis. PLoS Genet. 10, e1004365.
    1. Berrocal-Lobo, M., Molina, A. and Solano, R. (2002) Constitutive expression of ETHYLENE-RESPONSE-FACTOR1 in Arabidopsis confers resistance to several necrotrophic fungi. Plant J. 29, 23-32.
    1. Bohnert, H.J., Gong, Q.Q., Li, P.H. and Ma, S.S. (2006) Unraveling abiotic stress tolerance mechanisms - getting genomics going. Curr. Opin. Plant Biol. 9, 180-188.
    1. Bolt, S., Zuther, E., Zintl, S., Hincha, D.K. and Schmulling, T. (2017) ERF105 is a transcription factor gene of Arabidopsis thaliana required for freezing tolerance and cold acclimation. Plant, Cell Environ. 40(1), 108-120.

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