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. 2019 May 2;17(2):eAO4328.
doi: 10.31744/einstein_journal/2019AO4328.

Applicability of papain solutions in immunohematology

[Article in English, Portuguese]
Laís Priscila De Santis  1 , Patrícia Carvalho Garcia  1 , Valéria Nogueira Dias Paes Secco  1 , Rosana Rossi Ferreira  2 , Elenice Deffune  1
Affiliations

Applicability of papain solutions in immunohematology

[Article in English, Portuguese]
Laís Priscila De Santis et al. Einstein (Sao Paulo). .

Abstract
in English, Portuguese

Objective: To compare the enzyme activity of different presentations of papain solution to validate in-house preparations.

Methods: Two papain solutions were prepared, and the third presentation was a commercial solution. Tests were carried out with samples of red cells typed as weak RhD.

Results: In-house prepared papain solutions showed similar enzyme reactivity, and statistically no differences compared to the enzyme activity of the commercial solution.

Conclusion: Evaluating the cost-benefit ratio, the in-house prepared papain solutions present more economic advantages, and can be incorporated into immunohematological routines as a way to cope with periods of financial crisis and cost-containment policies.

Objetivo:: Comparar a atividade enzimática de diferentes apresentações de solução de papaína para validação de preparados in-house.

Métodos:: Foram preparadas duas soluções de papaína, e a terceira apresentação tratou-se de uma solução comercial. Os testes comparativos das reações enzimáticas foram realizados com amostras de hemácias tipadas como RhD fraco.

Resultados:: As soluções de papaína preparadas in-house apresentaram reatividade enzimática semelhante e estatisticamente sem diferenças em comparação com a atividade enzimática da solução comercial.

Conclusão:: Avaliando-se a relação entre custo e benefício, as soluções de papaína preparadas in-house são economicamente vantajosas, podendo ser incorporadas às rotinas imuno-hematológicas como forma de enfrentamento em períodos de crise financeira e em políticas de retenção de gastos.

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Conflict of interest statement

Conflict of interest:

none.

Figures

Figure 1
Figure 1. Preparation outline of the of the papain solutions
(A) Preparation of PAP1, as per protocol of the American Association of Blood Banks; (B) preparation of PAP2, as per French protocols.
Figure 2
Figure 2. Some agglutination reactions observed with weak RhD red blood cells, both untreated and treated with different solutions of papain. (A) reactivity of weak RhD samples without treatment; (B) reactivity of weak RhD samples treated with PAP1; (C) reactivity of weak RhD samples treated with PAP2; (D) reactivity of weak RhD samples treated with PAP3
See this image and copyright information in PMC

References

    1. Mohandas N, Gallagher PG. Red cell membrane: past, present, and future. Blood. 2008;112(10):3939–3948. Review. - PMC - PubMed
    1. Murador P, Deffune E. Aspectos estruturais da membrana eritrocitária. Rev Bras Hematol Hemoter. 2007;29(2):168–178. Review.
    1. Lux SE., 4th. Anatomy of the red cell membrane skeleton: unanswered questions. Blood. 2016;127(2):187–199. Review. - PubMed
    1. International Society of Blood Transfusion (ISBT) Table of blood group antigens [Internet] Amsterdam: ISBT; 2014. [[cited 2016 Nov 2]]. Available from: http://www.isbtweb.org/fileadmin/user_upload/files-2015/red%20cells/link....
    1. International Society of Blood Transfusion (ISBT) Table of blood group collections [Internet] Amsterdam: ISBT; 2014. [[cited 2016 Nov 2]]. Available from: http://www.isbtweb.org/fileadmin/user_upload/files-2015/red%20cells/link....

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