Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Jan 31;10(2):109-117.
doi: 10.1080/21501203.2019.1572034. eCollection 2019 Jun.

Bioprospecting potential and secondary metabolite profile of a novel sediment-derived fungus Penicillium sp. ArCSPf from continental slope of Eastern Arabian Sea

Arakkaveettil Kabeer Farha  1 , Abdulla Mohamed Hatha  1
Affiliations

Bioprospecting potential and secondary metabolite profile of a novel sediment-derived fungus Penicillium sp. ArCSPf from continental slope of Eastern Arabian Sea

Arakkaveettil Kabeer Farha et al. Mycology. .

Abstract

Marine fungi, one of the major decomposers of marine environment, is found to produce potential enzymes and novel biomolecules. The present study explored bioprospecting potentials such as antimicrobial, anticancer and enzymatic activities of marine sediment-derived fungi isolated from continental slope of Eastern Arabian Sea. Morphology and ITS sequencing identified the fungus as Penicillium sp. ArCSPf. The fungal strain exhibited amylase, gelatinase, phytase, lipase and pectinase activity. The active fraction obtained from the ethyl acetate extract column fractionation (F2) of fungus showed antibacterial activity against both methicillin-resistant Staphylococcus aureus (MRSA) and Bacillus cereus. Minimum inhibitory concentrations of F2 were 125 μg/mL for MRSA and 62.5 μg/mL for B. cereus. The active fraction showed a significant anticancer activity (IC50 = 22.79 µg/mL) against MCF-7 breast cancer cells. The secondary metabolite (Z)-Octadec-9-enamide (oleamide, m/z 282.27 (M + H+)] was identified in the LC-MS/MS analysis of active fraction F2 in positive ionisation mode. To the best of our knowledge, this is the first report on exploring the bioprospecting potential of a sediment-derived fungus from continental slope of eastern Arabian Sea for the production of therapeutically active compounds.

Keywords: Arabian Sea; LCMS; Penicillium; antibacterial; anticancer; continental slope; marine fungi; secondary metabolites.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Morphology of Penicillium sp. ArCSPf on different agar medium. (a) Czapek Dox agar. (b) Chloramphenicol-Rose Bengal agar. (c) Yeast Extract Peptone (YEPD) agar. (d) Sabouraud dextrose agar. (e) Microscopic image of Penicillium sp. ArCSPf. (f) Phylogenetic tree based on ITS1-5.8S-ITS2 sequences showing relationship between Penicillium sp. ArCSPf and related Penicillium species.
Figure 2.
Figure 2.
Bioprospecting potential of Penicillium sp. ArCSPf. (a) Antibacterial activity. (b) In-vitro cytotoxic analysis by MTT assay. (c) Morphology of MCF 7 cells before and after treatment with F2 fraction of Penicillium sp. ArCSPf.
Figure 3.
Figure 3.
(a) The total ion chromatograms (TIC) of the active fraction of Penicillium sp. ArCSPf. (b) MS–MS spectrum of oleamide.
See this image and copyright information in PMC

References

    1. Bonugli-Santos RC, dos Santos Vasconcelos MR, Passarini MRZ, Vieira GAL, Lopes VCP, Mainardi PH, dos Santos JA, de Azevedo Duarte L, Otero IVR, da Silva Yoshida AM, et al. 2015. Marine-derived fungi: diversity of enzymes and biotechnological applications. Front Microbial. 6:269. - PMC - PubMed
    1. Capon RJ, Stewart M, Ratnayake R, Lacey E, Gill JH.. 2007. Citromycetins and bilains A-C: new aromatic polyketides and diketopiperazines from Australian marine derived and terrestrial Penicillium spp. J Nat Prod. 70:1746–1752. - PubMed
    1. Deshmukh SK, Prakash V, Ranjan N.. 2017. Marine fungi: a source of potential anticancer compounds. Frontiers in Microbiol. 8:2536. - PMC - PubMed
    1. Devi JAI, Muthu AK. 2014. Gas chromatography-mass spectrometry analysis of bioactive constituents in the ethanolic extract of Saccharum spontaneum Linn. Int J Pharm Pharm Sci. 6:755–759.
    1. Devi P, Rodrigues C, Naik CG, D’Souza L. 2012. Isolation and characterization of antibacterial compound from a mangrove-endophytic fungus, Penicillium chrysogenum MTCC 5108. Indian J Microbiol. 52(4):617–623. - PMC - PubMed

LinkOut - more resources