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. 2019;12(3):434-439.
doi: 10.14202/vetworld.2019.434-439. Epub 2019 Mar 21.

Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina

Fernando A Bessone  1 , Maria Laura Soriano Pérez  1   2 , Gustavo Zielinski  1 , Marina Dibarbora  1   2 , M B Conde  1 , Javier Cappuccio  1   2 , Fabrisio Alustiza  1
Affiliations

Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina

Fernando A Bessone et al. Vet World. 2019.

Abstract

Background: Pasteurella multocida (Pm) is the causative agent of progressive atrophic rhinitis (PAR) and pneumonic pasteurellosis (PN) in pigs. Pm is a member of the porcine respiratory complex responsible for important economic loss in the pig industry.

Aim: This study aimed to characterize the Pm strains recovered from clinical cases of PN and PAR and to elucidate the antibiotic susceptibility profiles of the strains.

Materials and methods: Sixty strains were characterized molecularly by polymerase chain reaction to determine species-specific gene, capsular type (A or D), and toxin A production. The agar diffusion method was employed to evaluate antibiotic resistance profiles.

Results: We found that 65% of strains belonged to capsular type A or D, and 15% of those were positive to toxA gene. The antibiotic susceptibility profiles found were sensitive in decreasing order to: Enrofloxacin, ceftiofur (CTF), ampicillin, tilmicosin (TIL), florfenicol (FFN), spectinomycin (SPC), gentamicin, oxytetracycline (OTC), and trimethoprim-sulfamethoxazole (TMS). Strains were resistant in decreasing order to: Lincomycin (LIN), tylosin (TYL), erythromycin (ERY), TMS, SPC, OTC, FFN, TIL, and CTF.

Conclusion: The toxA gene was detected in many Pm isolates from pneumonic lungs. Capsule type A or D was the most frequently found among the collected isolates. LIN, TYL, and ERY are the drugs which showed higher percentages of resistant isolates.

Keywords: Pasteurella multocida; antibiotics susceptibility profiles; molecular characterization; pig.

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Figures

Figure-1
Figure-1
Electrophoresis gel of polymerase chain reaction products. Lane 1: CapD; Lane 2: KMT1; Lane 3: CapA; Lane 4: Negative control; Lane 5: CapD; Lane 6: Ladder (100bp).
Figure-2
Figure-2
Profiles of antibiotic susceptibility of Pasteurella multocida strains.
Figure-3
Figure-3
Dendrogram shows conglomerates analysis of antibiotic profiles of Pasteurella multocida from progressive atrophic rhinitis and pneumonic pasteurellosis cases.
See this image and copyright information in PMC

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