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. 2019;12(3):472-476.
doi: 10.14202/vetworld.2019.472-476. Epub 2019 Mar 30.

Marek's disease herpesvirus serotype 1 in broiler breeder and layer chickens in Malaysia

Iryanti Othman  1 , Erkihun Aklilu  1
Affiliations

Marek's disease herpesvirus serotype 1 in broiler breeder and layer chickens in Malaysia

Iryanti Othman et al. Vet World. 2019.

Abstract

Aim: This study aimed to investigate the occurrence of Marek's disease (MD) in five poultry farms in Malaysia using postmortem examination, histopathology, and polymerase chain reaction (PCR).

Materials and methods: Tissue samples were collected from 24 broiler breeder chickens from four commercial broiler breeder farms and six layer chickens from one layer farm. Gross and histopathological examinations and PCR amplification of the gene encoding for avian MD herpesvirus (MDV-1) were conducted.

Results: Gross pathological changes including hepatomegaly, splenomegaly, lymphomatous lesion at the mesentery, oviduct atrophy, and follicular atresia with lymphomatous were observed, whereas diffuse multifocal whitish infiltration of the spleen, neoplastic infiltration in the liver, intrafollicular lymphoid infiltration of the bursa of Fabricius, and lymphomatous tumor at the mesentery were seen on histopathological examinations. Confirmation by PCR showed that a total of 16 (53.33%) samples were positive for avian MDV-1. Although the outbreak involved a much larger number of birds in the respective farms, our investigation was limited based on resource and time frame allocated for the study.

Conclusion: The findings from this study help in emphasizing the potential threats of MDV to the poultry industry globally, in general, and in Malaysia, in particular. As the scope of the current study is limited, future studies focusing on MDV pathogenesis, typing, and causes of vaccine failures are recommended.

Keywords: Marek’s disease; Marek’s disease virus-1; lymphoproliferative diseases; molecular detection; poultry diseases.

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References

    1. Van Regenmortel M.H.V, Fauquet C.M, Bishop D.H.L, Carsten E.B, Estes M.K, Lemon S.M, Maniloff J, Mayo M, McGeoch D.J, Pringle C.R, Wickner R.B. Virus Taxonomy:Seventh Report of the International Committee on Taxonomy of Viruses. New York: Academic Press; 2000.
    1. Venugopal N. Evolution of Marek's disease-a paradigm for incessant race between the pathogen and the host. Vet. J. 2005;170(2):175–183. - PubMed
    1. Witter R.L, Schat K.A. Marek's disease. In: Saif Y.M, Barness H.J, Glisson J.R, Fadly A.M, McDougald L.R, Swayne D.E, editors. Diseases of Poultry. 11th ed. Ames, Iowa, USA: Iowa State University Press; 2003. pp. 407–465.
    1. OIE. Manual of Diagnostic and Vaccine for Terrestrial Animals. Version Adopted by the World Assembly of Delegates of the OIE in May 2010. Paris, France: World Health Organization for Animal Health (OIE); 2010.
    1. Baigent S.J, Davison F. Marek's disease virus:Biology and life cycle. In:Marek's Disease:An Evolving Problem. London, United Kingdom: Elsevier Academic Press; 2004. pp. 62–77.

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