Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Clinical Trial
. 2019 Sep;311(7):535-544.
doi: 10.1007/s00403-019-01931-y. Epub 2019 May 14.

Narrowband UVB treatment induces expression of WNT7B, WNT10B and TCF7L2 in psoriasis skin

Malin Assarsson  1 , Jan Söderman  2 , Albert Duvetorp  3 , Ulrich Mrowietz  4 , Marita Skarstedt  2 , Oliver Seifert  5   6
Affiliations
Clinical Trial

Narrowband UVB treatment induces expression of WNT7B, WNT10B and TCF7L2 in psoriasis skin

Malin Assarsson et al. Arch Dermatol Res. 2019 Sep.

Abstract

WNT/β-catenin signaling pathways play a pivotal role in the human immune defense against infections and in chronic inflammatory conditions as psoriasis. Wnt gene alterations are linked to known comorbidities of psoriasis as obesity, diabetes and Crohn's disease. The objective of this study was to investigate WNT7B, WNT10B, WNT16 and TCF7L2 gene and protein expression in lesional and non-lesional skin and in the peripheral blood of patients with chronic plaque psoriasis compared with healthy individuals. To investigate the effect of narrowband UVB radiation, expression of these genes were analyzed before and after narrowband UVB treatment. Associations between single nucleotide polymorphisms for WNT7B, WNT10B, WNT16 and TCF7L2 genes and psoriasis were tested. Our results show significantly decreased WNT7B, WNT10B and TCF7L2 gene expression in lesional skin compared with non-lesional skin and healthy controls. Narrowband UVB treatment significantly increased expression of these genes in lesional skin. Immunohistochemistry shows increased WNT16 expression in lesional skin. No significant differences in allele or genotype frequencies for Wnt or TCF7L2 gene polymorphisms were found between patient and control group. This study shows for the first time significant UVB induced upregulation of WNT7B, WNT10B and TCF7L2 in patients with psoriasis and suggests a potential role of these genes in psoriasis pathogenesis.

Keywords: Gene expression; Inflammation; Single nucleotide polymorphisms; WNT proteins; WNT/β-catenin signaling.

PubMed Disclaimer

Conflict of interest statement

The authors have declared no conflicting interests.

Figures

Fig. 1
Fig. 1
Illustration of patient flow and number of patients with chronic plaque psoriasis (n) and healthy control individuals (c) included in the study and the different WNT7B, WNT10B, WNT16 and TCF7L2 analysis performed (nb narrowband, IHC immunohistochemistry)
Fig. 2
Fig. 2
Significantly decreased WNT7B (a), WNT10B (b) and TCF7L2 (c) gene expression in lesional compared with non-lesional skin in patients with psoriasis and significantly decreased WNT7B (a) and WNT10B (b) in lesional skin of patients with psoriasis compared with healthy controls (control n = 20, non-lesional and lesional n = 32, •mean, box = mean ± confidence interval, whiskers = mean ± SD, ***p < 0.001)
Fig. 3
Fig. 3
Narrowband UVB treatment significantly induces WNT7B (a), WNT10B (b) and TCF7L2 (c) gene expression in lesional skin of patients with psoriasis (pre = before and post = after nbUVB treatment, n = 27, •mean, box = mean ± 0.95 confidence interval, whiskers = mean ± SD, *p < 0.05, **p < 0.01 and ***p < 0.001)
Fig. 4
Fig. 4
Expression of WNT7B (a), WNT10B (b), WNT16 (c) and TCF7L2 (d) protein in skin from healthy controls (I), in non-lesional (II) and lesional skin (III) from patients with psoriasis analyzed by immunohistochemistry. Histology from one representative patient is shown (hematoxylin, n = 4, c = 4, 20 × magnification)
See this image and copyright information in PMC

References

    1. Abiola M, Favier M, Christodoulou-Vafeiadou E, Pichard AL, Martelly I, Guillet-Deniau I. Activation of Wnt/beta-catenin signaling increases insulin sensitivity through a reciprocal regulation of Wnt10b and SREBP-1c in skeletal muscle cells. PLoS One. 2009;4:e8509. doi: 10.1371/journal.pone.0008509. - DOI - PMC - PubMed
    1. Alok A, Lei Z, Jagannathan NS, et al. Wnt proteins synergize to activate beta-catenin signaling. J Cell Sci. 2017;130:1532–1544. doi: 10.1242/jcs.198093. - DOI - PubMed
    1. Andersen CL, Jensen JL, Orntoft TF. Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 2004;64:5245–5250. doi: 10.1158/0008-5472.CAN-04-0496. - DOI - PubMed
    1. Barrett JC, Fry B, Maller J, Daly MJ. Haploview: analysis and visualization of LD and haplotype maps. Bioinformatics. 2005;21:263–265. doi: 10.1093/bioinformatics/bth457. - DOI - PubMed
    1. Bennett CN, Longo KA, Wright WS, et al. Regulation of osteoblastogenesis and bone mass by Wnt10b. Proc Natl Acad Sci USA. 2005;102:3324–3329. doi: 10.1073/pnas.0408742102. - DOI - PMC - PubMed

Publication types

MeSH terms