Muricauda nanhaiensis sp. nov., isolated from seawater of the South China Sea

Abstract

A Gram-stain-negative, aerobic, oxidase- and catalase-positive, non-flagellated, non-gliding, yellow-pigmented, and rod-shaped bacterium with appendages, designated strain SM1704^T, was isolated from surface seawater collected from the South China Sea. The strain grew at 15-42 °C and with 1-10 % NaCl. It hydrolysed aesculin, but did not hydrolyse gelatin and Tween 80 nor reduce nitrate to nitrite. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SM1704^T was affiliated with the genus Muricauda, sharing 94.1-95.9 % sequence similarities with type strains of recognized Muricauda species. The major fatty acids were iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0 3-OH and the main polar lipids were phosphatidylethanolamine, three unidentified lipids and three unidentified aminolipids. The major respiratory quinone was menaquinone-6. The genomic DNA G+C content of strain SM1704^T was 40.7 mol%. On the basis of results from polyphasic analysis of strain SM1704^T, it is considered to represent a novel species within the genus Muricauda, for which the name Muricaudananhaiensis sp. nov. is proposed. The type strain is SM1704^T (=KCTC 62797^T=MCCC 1K03557^T).

Keywords: Flavobacteriaceae; Muricauda; South China Sea; sp. nov..