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. 2019 Aug;86(2):202-207.
doi: 10.1038/s41390-019-0430-8. Epub 2019 May 15.

Expression of (pro)renin receptor and its effect on endothelial cell proliferation in infantile hemangioma

Bede van Schaijik  1 , Swee T Tan  2   3 , Reginald W Marsh  1   4 , Tinte Itinteang  1
Affiliations

Expression of (pro)renin receptor and its effect on endothelial cell proliferation in infantile hemangioma

Bede van Schaijik et al. Pediatr Res. 2019 Aug.

Abstract

Background: Propranolol is the preferred treatment for problematic proliferating infantile hemangioma (IH) by targeting the renin-angiotensin system (RAS) expressed by IH endothelium. (Pro)renin receptor (PRR) is a major component of the RAS associated with the canonical wnt signaling pathway. We proposed that activation of PRR by renin causes proliferation of IH.

Methods: The expression of PRR in IH tissue samples was investigated using immunohistochemical (IHC) staining and NanoString analysis. NanoString analysis was also used to confirm transcriptional expression of PRR in CD34-sorted proliferating IH-derived primary cell lines. MTT assay was utilized to determine the effect of exogenous renin on the number of viable IH cells. RT-qPCR was used to determine the effect of renin on the stem cell gene expression.

Results: NanoString analysis and IHC staining confirmed transcriptional and translational expression of PRR, which was localized to the non-endothelial and the endothelial IH cell populations. MTT assay demonstrated an increased number of viable IH cells by administration of renin and the effect was negated by the wnt receptor blocker dickkopf-1.

Conclusion: Our results present a model for renin-induced increased proliferation of IH cells through PRR acting via the wnt signaling pathway, which may account for accumulation of cells in IH during the proliferative phase of the tumor.

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References

    1. Itinteang, T., Brasch, H. D., Tan, S. T. & Day, D. J. Expression of components of the renin-angiotensin system in proliferating infantile haemangioma may account for the propranolol-induced accelerated involution. J. Plast. Reconstr. Aesthet. Surg. 64, 759–765 (2011). - DOI
    1. Tan, S. T. et al. A novel in vitro human model of hemangioma. Mod. Pathol. 13, 92–99 (2000). - DOI
    1. North, P. E. et al. A unique microvascular phenotype shared by juvenile hemangiomas and human placenta. Arch. Dermatol 137, 559–570 (2001). - DOI
    1. Takahashi, K. et al. Cellular markers that distinguish the phases of hemangioma during infancy and childhood. J. Clin. Invest 93, 2357–2364 (1994). - DOI
    1. Itinteang, T. et al. Infantile haemangioma expresses embryonic stem cell markers. J. Clin. Pathol. 65, 394–398 (2012). - DOI

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