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Published Erratum
. 2019 May 15;38(10):e101812.
doi: 10.15252/embj.2019101812.

Reactive oxygen species are essential for autophagy and specifically regulate the activity of Atg4

Ruth Scherz-Shouval, Elena Shvets, Ephraim Fass, Hagai Shorer, Lidor Gil, Zvulun Elazar
Published Erratum

Reactive oxygen species are essential for autophagy and specifically regulate the activity of Atg4

Ruth Scherz-Shouval et al. EMBO J. .

Abstract

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Figures

Figure 7A
Figure 7A. Updated legend to Figure 7A
Mutation in Cys81 reduces the redox sensitivity of HsAtg4A in vitro. (A) Recombinant His6‐HsAtg4AWT, His6‐HsAtg4AC77A, or His6‐HsAtg4AC81S (0.1 μg) was incubated with His6‐GATE‐16‐HA (0.3 μg) in 50 KT reaction buffer at 30°C for 45 min in the presence or absence of 0.1 or 1 mM DTT, as indicated. Reaction mixtures were analyzed by Western blot using anti‐His monoclonal antibodies. (*) indicates non‐cleaved His6‐GATE‐16‐HA and (**) indicates cleaved His6‐GATE‐16.
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