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. 2019 May 16;14(5):e0214494.
doi: 10.1371/journal.pone.0214494. eCollection 2019.

Mueller-matrix-based polarization imaging and quantitative assessment of optically anisotropic polycrystalline networks

Mariia Borovkova  1 , Larysa Trifonyuk  2 , Volodymyr Ushenko  3 , Olexander Dubolazov  4 , Oleg Vanchulyak  5 , George Bodnar  5 , Yurii Ushenko  6 , Olena Olar  6 , Olexander Ushenko  4 , Michael Sakhnovskiy  4 , Alexander Bykov  1 , Igor Meglinski  1   7   8
Affiliations

Mueller-matrix-based polarization imaging and quantitative assessment of optically anisotropic polycrystalline networks

Mariia Borovkova et al. PLoS One. .

Abstract

We introduce a Mueller-matrix imaging polarization-based approach for the quantitative digital screening of the polycrystalline structure of fibrillary-based biological tissues in vitro. The morphometric evaluation of histological sections of myocardium was performed utilizing the high-order statistical moments calculated based on the spatial distribution of linear and circular birefringence and dichroism obtained experimentally. We demonstrate that spatial distributions of phase of light and optical anisotropy of scattering inherent to fibrillar networks of myocardium at different necrotic stages can be effectively used as a quantitative marker of stages of myosin fibril degradation. Processing the images of phase of light scattered in biological tissues with high order statistical analysis provides a functional tool for the quantitative characterization of necrotic conditions of the myocardium.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Schematic presentation of the Mueller-polarimeter experimental setup, including “blue” laser diode (1), collimator (2), stationary quarter-wave plate (3), mechanically movable quarter-wave plates (5) and (8), polarizer (4) and analyzer (9), object of investigation (6), infinity corrected strain-free objective with tube lens (7), CCD camera (10), personal computer (11).
Fig 2
Fig 2
Microscopic (4x) polarization-visualized images of the optically anisotropic component of the histological sections of the myocardium of control (a) and diagnosed (b) groups.
Fig 3
Fig 3. Polarization-phase spatial distribution PT(m×n) and histograms N(PT) of the distributions Φ(m×n) and A(m×n) of myocardium histological sections of deceased patients from the group 1 (Δn0;90, Δn⊗;⊕, Δμ0;90, Δμ⊗;⊕) and the group 2 (Δn0;90*, Δn⊗;⊕*, Δμ0;90*, Δμ⊗;⊕*).
See this image and copyright information in PMC

References

    1. Tuchin V, Wang L, Zimnjakov D. Optical polarization in biomedical applications. New York, USA: Springer; 2006.
    1. Ghosh N, Wood M, Vitkin A. Polarized light assessment of complex turbid media such as biological tissues via Mueller matrix decomposition In: Tuchin V, editors. Handbook of Photonics for Biomedical Science. London: CRC Press, Taylor & Francis Group; 2010. pp. 253–282.
    1. Jacques S. Polarized light imaging of biological tissues In: Boas D, Pitris C, Ramanujam N, editors. Handbook of Biomedical Optics. Boca Raton, London, New York: CRC Press; 2011. pp. 649–669.
    1. Ghosh N. Tissue polarimetry: concepts, challenges, applications, and outlook. J. Biomed. Opt. 2011;16(11):110801 10.1117/1.3652896 - DOI - PubMed
    1. Layden D, Ghosh N, Vitkin A. Quantitative polarimetry for tissue characterization and diagnosis In: Wang R, Tuchin V, editors. Advanced Biophotonics: Tissue Optical Sectioning. Boca Raton, London, New York: CRC Press, Taylor & Francis Group; 2013. pp. 73–108.

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