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. 2019 Mar-Apr;23(2):134-138.
doi: 10.1016/j.bjid.2019.04.005. Epub 2019 May 16.

Molecular characteristics of methicillin-resistant Staphylococcus aureus isolates from hospital and community environments in northeastern Brazil

Suzi P de Carvalho  1 , Jéssica B de Almeida  2 , Yasmin M F S Andrade  2 , Lucas S C da Silva  2 , Raiane C Chamon  3 , Kátia R N Dos Santos  4 , Lucas M Marques  5
Affiliations

Molecular characteristics of methicillin-resistant Staphylococcus aureus isolates from hospital and community environments in northeastern Brazil

Suzi P de Carvalho et al. Braz J Infect Dis. 2019 Mar-Apr.

Abstract

This study characterized 30 MRSA isolates from intensive care unit (ICU) environment and equipment surfaces and healthy children. The SCCmec types I, IVa and V were detected in HA-MRSA isolates while CA-MRSA showed the SCCmec type IVa and V. Most isolates were classified as agr group II. All isolates presented the sei gene, and only HA-MRSA were positive for etb e tst genes. Three genotypes were related to Pediatric (ST5/SCCmecIV) and Berlin (ST45/SCCmecIV) clones. The present study showed molecular similarity between CA- and HA-MRSA isolates in hospital and community settings in a Brazilian region.

Keywords: CA-MRSA; HA-MRSA; Methicillin-resistant Staphylococcus aureus; Molecular typing.

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Figures

Fig. 1
Fig. 1
Dendrogram of the PFGE patterns related to 30 CA- and HA-MRSA isolates. Isolates showing a similarity coefficient ≥80% were considered genetically related. DCC, day care centers. One isolate, S. aureus strain ATCC 29213/ST5 was used as control. aRelated to USA600 lineage; bRelated to USA800 lineage.
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References

    1. de Sousa M.A., Conceicao T., Simas C., De Lencastre H. Comparison of genetic backgrounds of methicillin-resistant and-susceptible Staphylococcus aureus isolates from Portuguese hospitals and the community. J Clin Microbiol. 2005;43:5150–5157. - PMC - PubMed
    1. Caboclo R.M.F., Cavalcante F.S., Iorio N.L.P., et al. Methicillin-resistant Staphylococcus aureus in Rio de Janeiro hospitals: dissemination of the USA400/ST1 and USA800/ST5 SCCmec type IV and USA100/ST5 SCCmec type II lineages in a public institution and polyclonal presence in a private one. Am J Infect Contr. 2013;41:e21–e26. - PubMed
    1. Schuenck R.P., Nouér S.A., de Oliveira Winter C., et al. Polyclonal presence of non-multiresistant methicillin-resistant Staphylococcus aureus isolates carrying SCCmec IV in health care-associated infections in a hospital in Rio de Janeiro, Brazil. Diag Microbiol Infect Dis. 2009;64:434–441. - PubMed
    1. Zhang K., McClure J.-A., Elsayed S., Louie T., Conly J.M. Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. J Clin Microbiol. 2005;43:5026–5033. - PMC - PubMed
    1. Gilot P., Lina G., Cochard T., Poutrel B. Analysis of the genetic variability of genes encoding the RNA III-activating components Agr and TRAP in a population of Staphylococcus aureus strains isolated from cows with mastitis. J Clin Microbiol. 2002;40:4060–4067. - PMC - PubMed

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