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. 2018 Oct 20;8(20):e3056.
doi: 10.21769/BioProtoc.3056.

Murine Pharmacokinetic Studies

Alix F Leblanc  1 , Kevin M Huang  1 , Muhammad Erfan Uddin  1 , Jason T Anderson  1 , Mingqing Chen  1 , Shuiying Hu  1
Affiliations

Murine Pharmacokinetic Studies

Alix F Leblanc et al. Bio Protoc. .

Abstract

Murine pharmacokinetics (PK) represents the absorption, distribution, metabolism, and elimination of drugs from the body, which helps to guide clinical studies, ultimately resulting in more effective drug treatment. The purpose of this protocol is to describe a serial bleeding protocol, obtaining blood samples at six time points from single mouse to yield a complete PK profile. This protocol has proved to be rapid, highly repeatable, and relatively easy to acquire. Comparing with the conventional PK studies, this method not only dramatically reduces animal usage, but also decreases sample variation obtained from different animals.

Keywords: Blood; Cardiac puncture; Orbital venous plexus; Pharmacokinetic; Plasma; Submandibular vein.

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Conflict of interest statement

Competing interests The authors do not have any potential conflicts of interest to declare.

Figures

Video 1.
Video 1.. Blood collection platform
Figure 1.
Figure 1.. Time scheme of drug administration and blood collection
Figure 2.
Figure 2.. Visualized landmark for mice cheek bleeding
Video 2.
Video 2.. Cheek bleeding
(This video was made at The Ohio State University according to guidelines from The Ohio State University on Animal Care and Use Committee of The Ohio State University, and approved by the Animal Research Ethics Board of The Ohio State University under protocol #2015A10000101.)
Video 3.
Video 3.. Retro Orbital bleeding
(This video was made at The Ohio State University according to guidelines from The Ohio State University on Animal Care and Use Committee of The Ohio State University, and approved by the Animal Research Ethics Board of The Ohio State University under protocol #2015A10000101.)
Video 4.
Video 4.. Cardiac puncture
(This video was made at The Ohio State University according to guidelines from The Ohio State University on Animal Care and Use Committee of The Ohio State University, and approved by the Animal Research Ethics Board of The Ohio State University under protocol #2015A10000101).
Video 5.
Video 5.. Blood process platform
Figure 3.
Figure 3.. Plasma concentration-time curves of paclitaxel after paclitaxel administration (10 mg/kg; i.v.) pretreated with nilotinib (100 mg/kg; p.o.) or vehicle (n = 5 per group)
See this image and copyright information in PMC

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