[Localization of lysine residues in the site of initiating substrate binding of E. coli RNA-polymerase]
[Article in
Russian]
- PMID: 3111488
Item in Clipboard
[Localization of lysine residues in the site of initiating substrate binding of E. coli RNA-polymerase]
[Article in
Russian]
Bioorg Khim.
1987 Apr.
Abstract
Superselective affinity labelling of E. coli RNA polymerase in a complex with the promoter-containing fragment of T7 DNA by treatment with orto-formylphenyl ester of GMP followed by addition of [alpha-33P]UTP resulted in covalent binding of the residue--pGpU (p-radioactive phosphate) with one of lysine residues of the beta-subunit, Lys1048, Lys1051, Lys1057, Lys1065. The amino acid sequence of this region of the beta-subunit of E. coli RNA polymerase has a high extent of homology with that deduced for a region of tobacco chloroplast RNA polymerase on the basis of the nucleotide sequence of the chloroplast rpoB-like gene.
Similar articles
-
[Localization of a lysine residue near the site of initiating substrate binding of T7 bacteriophage RNA polymerase].Bioorg Khim. 1989 Jan;15(1):18-23. Bioorg Khim. 1989. PMID: 2500935 Russian.
-
[Highly selective labeling of the E. coli RNA-polymerase promoter complex with reactive derivatives of oligonucleotide primers of various specificity].Bioorg Khim. 1990 Jun;16(6):765-79. Bioorg Khim. 1990. PMID: 2222526 Russian.
-
[Localization of a histidine residue in the binding site for the initiating substrate of E. coli RNA-polymerase].Bioorg Khim. 1987 Jul;13(7):992-5. Bioorg Khim. 1987. PMID: 3314873 Russian.
-
[Affinity modification of E. coli RNA-polymerase in a complex with the promoter by phosphorylating derivatives of primer oligonucleotides].Bioorg Khim. 1986 Dec;12(12):1678-81. Bioorg Khim. 1986. PMID: 3545219 Russian.
-
[Localization of binding sites of E. coli DNA-dependent RNA-polymerase with photosensitive template analogs].Bioorg Khim. 1986 Aug;12(8):1023-9. Bioorg Khim. 1986. PMID: 3535808 Russian.
Cited by
-
Primary structure and functional aspects of the gene coding for the second-largest subunit of RNA polymerase III of Drosophila.Mol Gen Genet. 1989 Nov;219(3):373-80. doi: 10.1007/BF00259609. Mol Gen Genet. 1989. PMID: 2482932
-
Relatedness of archaebacterial RNA polymerase core subunits to their eubacterial and eukaryotic equivalents.Nucleic Acids Res. 1988 Aug 25;16(16):8113-28. doi: 10.1093/nar/16.16.8113. Nucleic Acids Res. 1988. PMID: 2843811 Free PMC article.
-
The A14-A43 heterodimer subunit in yeast RNA pol I and their relationship to Rpb4-Rpb7 pol II subunits.Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14670-5. doi: 10.1073/pnas.232580799. Epub 2002 Oct 29. Proc Natl Acad Sci U S A. 2002. PMID: 12407181 Free PMC article.