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. 2019 Jan-Mar;14(1):95-105.

Histopathological and Molecular Evaluation of the Experimentally Infected Goats by the Larval Forms of Taenia multiceps

Omidreza Amrabadi  1 , Ahmad Oryan  1 , Mohammad Moazeni  2 , Hassan Shari-Fiyazdi  3 , Maryam Akbari  2
Affiliations

Histopathological and Molecular Evaluation of the Experimentally Infected Goats by the Larval Forms of Taenia multiceps

Omidreza Amrabadi et al. Iran J Parasitol. 2019 Jan-Mar.

Abstract

Background: Introduction of Taenia multiceps and T. gaigeri as two separate species have been recognized mainly on morphological grounds. This experimental study was conducted to test whether cerebral and non-cerebral forms of Coenurus cerebralis belong to one origin or they are originated from two different tape worms.

Methods: Two groups of dogs were infected with the cerebral and muscular sources of the coenuri cysts. About two months later the eggs were collected from the fecal samples to be used to experimentally infect other healthy goats. Histopathological and molecular evaluation was conducted in two groups of goats that were challenged with T. multiceps eggs obtained from the infected dogs by brain and muscular sources of coenuri cysts in School of Veterinary Medicine of Shiraz University, Shiraz, Iran in 2015. All aberrant sites of predilection of the metacestode in goats were muscles, heart, diaphragm and lungs. The brain and spinal cord were carefully dissected and examined but the cysts were not found in these locations. In addition, the molecular genetic markers of mitochondrial DNA (CO1 and ND1) were applied to resolve the questionable relationship between T. multiceps and T. gaigeri.

Results: The larval stages of T. multiceps in brain and in other aberrant sites, which showed similar morphological criteria, were monophyletic species.

Conclusion: Therefore, T. gaigeri must be considered taxonomically invalid.

Keywords: Coenurosis; Coenurus cerebralis; DNA (CO1 and ND1); Taenia gaigeri; Taenia multiceps.

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Conflict of interest statement

Conflict of interest The authors declare no conflict of interest.

Figures

Fig. 1:
Fig. 1:
Jejunum of a dog heavily infected with large number of cestodes after four months post infection
Fig. 2:
Fig. 2:
The submucosa of jejunum shows prominent cellular infiltration mainly consisting of numerous eosinophils after 56 d post infection. (HE, scale bar = 85μm). Inset: large numbers of eosinophils are seen (arrow) (× 400)
Fig. 3:
Fig. 3:
a) Apical lobe of the lung with a coenurus cyst in an experimentally infected goat infected by the eggs of muscle source T. multiceps four months post infection. b) Several coenuri cysts have invaded the heart of an experimentally infected goat infected by the eggs of muscle source T. multiceps four months post infection. c) Three coenuri cysts are embedded between the muscles of right thigh of an experimentally infected goat infected by the eggs of muscle source T. multiceps 12 months post infection. d) Section from the coenurus cyst in the lung four months post infection showing the metacestode wall (arrow) (HE, scale bar = 670 μm). Inset: infiltration of lymphocytes and proliferation of fibroblasts (asterisk) together with atelectasis of the alveoli and air passages is seen (arrowhead) (×40)
Fig. 4:
Fig. 4:
a) Photomicrograph of cardiac tissue of an infected goat showing early developing coenurus cyst with its premature protoscoleces (arrow) four months post infection. The cyst is surrounded by mononuclear cell infiltration (HE, scale bar = 210 μm). Inset: infiltration of lymphocytes, macrophages and eosinophils are seen (arrowhead) (×400). b) The arrowhead shows sucker of the larvae within the coenuri cyst four months post infection. The cyst has a thin wall (large arrow) and is surrounded by mononuclear cell infiltration (HE, scale bar = 210 μm). Inset: infiltration of lymphocytes is seen (small arrow) (×400). c) Granulomatus myositis due to coenurus larvae of an experimentally infected goat infected by the eggs of muscle source T. multiceps, 12 months post infection, with central caseous necrosis surrounded by eosinophils, lymphocytes, epitheloid macrophages and giant cells (arrow) (HE, scale bar = 21 μm). d) A coenurus cyst embedded between the intercostal muscles of a goat experimentally infected by the eggs of brain source T. multiceps four months post infection
Fig. 5:
Fig. 5:
a) Partial Alignment of the CO1 sequences for T. multiceps strains B and M compared to other strains existing in GenBank. b) Partial Alignment of the ND1 sequences for T. multiceps strains B and M compared to other strains existing in GenBank
See this image and copyright information in PMC

References

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