Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Apr 26;6(5):ofz187.
doi: 10.1093/ofid/ofz187. eCollection 2019 May.

A Comparison of Stool Enteropathogen Detection by Semiquantitative PCR in Adults With Acute Travelers' Diarrhea Before and 3 Weeks After Successful Antibiotic Treatment

Michele D Tisdale  1   2   3 , David R Tribble  1 , Kalyani Telu  1   2 , Jamie A Fraser  1   2 , Patrick Connor  4 , Cliff Philip  5 , Elizabeth Odundo  5 , Faviola Reyes  6 , Mark P Simons  7 , Brett Swierczewski  8 , Stephen Lizewski  9 , Jie Liu  10 , Eric Houpt  10 , Mark S Riddle  11 , Tahaniyat Lalani  1   2   3
Affiliations

A Comparison of Stool Enteropathogen Detection by Semiquantitative PCR in Adults With Acute Travelers' Diarrhea Before and 3 Weeks After Successful Antibiotic Treatment

Michele D Tisdale et al. Open Forum Infect Dis. .

Abstract

We evaluated stool enteropathogen detection by semiquantitative polymerase chain reaction (PCR) in 108 subjects with travelers' diarrhea before and 3 weeks after treatment. Stool samples from 21 subjects were positive for the same pathogen species at both visits. We discuss factors that should be considered when interpreting stool PCR data after treatment.

Keywords: PCR; infectious disease; military; travelers’ diarrhea.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Distribution of pathogens in the 108 paired stool samples from subjects with travelers’ diarrhea collected at day 0 before antibiotic therapy and day 21 after treatment.a aNo samples were positive for Aeromonas, Astrovirus, Campylobacter coli, Cyclospora, Entameoba histolytica, Rotavirus, Salmonella, Vibrio parahemolyticus. bRepresents all samples positive at day 0 (regardless of positivity at day 21). Abbreviations: EIEC, enteroinvasive Escherichia coli; EPEC, enteropathogenic E. coli; LT, heat-labile; ST, shiga toxin; STEC, shiga toxin–producing E. coli.
See this image and copyright information in PMC

References

    1. Lääveri T, Antikainen J, Pakkanen SH, et al. . Prospective study of pathogens in asymptomatic travellers and those with diarrhoea: aetiological agents revisited. Clin Microbiol Infect 2016; 22:535–41. - PubMed
    1. Paschke C, Apelt N, Fleischmann E, et al. . Controlled study on enteropathogens in travellers returning from the tropics with and without diarrhoea. Clin Microbiol Infect 2011; 17:1194–200. - PubMed
    1. Lertsethtakarn-Ketwalha P, Silapong S, Sakpaisal P, et al. . Travelers’ diarrhea in Thailand: a quantitative analysis using TaqMan(R) array card. Clin Infect Dis 2018;. 67(1):120–7. - PMC - PubMed
    1. Connor BA, Rogova M, Whyte O. Use of a multiplex DNA extraction PCR in the identification of pathogens in travelers’ diarrhea. J Travel Med 2017; 25:1–6. - PubMed
    1. Sethabutr O, Venkatesan M, Murphy GS, et al. . Detection of Shigellae and enteroinvasive Escherichia coli by amplification of the invasion plasmid antigen H DNA sequence in patients with dysentery. J Infect Dis 1993; 167:458–61. - PubMed