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. 2019:2008:73-81.
doi: 10.1007/978-1-4939-9537-0_6.

Antibody-Driven Proximity Labeling in Fixed Tissues

Daniel Z Bar  1   2 , Francis S Collins  3
Affiliations

Antibody-Driven Proximity Labeling in Fixed Tissues

Daniel Z Bar et al. Methods Mol Biol. 2019.

Abstract

Protein function often depends on assemblies and interactions. These show complex spatial and temporal organization within the cell. Analysis of protein function can be greatly assisted by grouping proteins with their neighbors. Rather than relying on affinity, proximity labeling targets proteins proximal to the target of interest. We describe a protocol for antibody-guided deposition of tags in fixed and permeabilized cell lines and primary human tissue samples.

Keywords: Antibody; Biotin; HRP; Mass-spectrometry; Protein-protein interactions; Proximity labeling.

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Figures

Fig. 1
Fig. 1
Outline of the antibody-driven proximity labeling method
See this image and copyright information in PMC

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