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. 2019;17(1):40-46.
doi: 10.2174/1871525717666190524092918.

Radioprotective Effect of Gliclazide as an Anti-Hyperglycemic Agent Against Genotoxicity Induced by Ionizing Radiation on Human Lymphocytes

Maysa Pouri  1   2 , Zahra Shaghaghi  1   3 , Arash Ghasemi  4 , Seyed Jalal Hosseinimehr  1
Affiliations

Radioprotective Effect of Gliclazide as an Anti-Hyperglycemic Agent Against Genotoxicity Induced by Ionizing Radiation on Human Lymphocytes

Maysa Pouri et al. Cardiovasc Hematol Agents Med Chem. 2019.

Abstract

Objectives: Gliclazide (GL) is widely used to reduce hyperglycemia in diabetic patients. The aim of this study was to investigate the protective effect of GL against chromosome damage induced by ionizing radiation in human blood lymphocytes.

Methods: In this experimental study, peripheral blood samples were collected from human volunteers and treated with GL at various concentrations (5, 25, 50 or 100 μM) for three hours. Then samples were irradiated to X-ray (1.5 Gy). Blood samples were cultured with mitogenic stimulation. The frequencies of micronuclei in cytokinesis-blocked binucleated lymphocytes were determined in the different samples. The antioxidant activities of GL were assayed by two different methods as 1,1- diphenyl-2-picryl hydrazyl radical (DPPH) free radical scavenging and reducing antioxidant power assays.

Results: GL significantly reduced the percentage of micronuclei in lymphocytes which were irradiated. The maximum radioprotection in the reduction of percentage of micronuclei in lymphocytes was observed at 100 μM of GL with 52% efficacy. GL exhibited excellent free radical scavenging activity and reducing power at concentration dependent activities. The IC50 values of GL were lower than ascorbic acid. Higher potencies were observed in the antioxidant activities for GL than ascorbic acid in both methods.

Conclusion: This data exhibits that GL is a powerful radioprotective agent that could protect healthy cells against the chromosome damage induced by ionizing radiation through antioxidant activity. The radioprotective effect is new indication of GL for patient's protection against side effect induced by ionizing radiation.

Keywords: DNA; genotoxicity; gliclazide; ionizing radiation; radioprotective; reactive oxygen species..

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Figures

Fig. (1)
Fig. (1)
A typical binucleated lymphocyte with micronucleus in this study.
Fig. (2)
Fig. (2)
Free radical scavenging effect of different concentrations of gliclazide and ascorbic acid on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical at 517 nm.
Fig. (3)
Fig. (3)
Reducing power of different concentrations of gliclazide and ascorbic acid with ferric reducing power system.
See this image and copyright information in PMC

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