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Comparative Study
. 2019 May 23;90(2):241-247.
doi: 10.23750/abm.v90i2.6939.

Comparative study on anti-proliferative potentials of zinc oxide and aluminium oxide nanoparticles in colon cancer cells

Vimala Devi Subramaniam  1 , Murugesan Ramachandran  1 , Francesco Marotta  2 , Antara Banerjee  1 , Xiao Feng Sun  3 , Surajit Pathak  1
Affiliations
Comparative Study

Comparative study on anti-proliferative potentials of zinc oxide and aluminium oxide nanoparticles in colon cancer cells

Vimala Devi Subramaniam et al. Acta Biomed. .

Abstract

Background and aim of the study: Use of commercial products containing nanoparticles formulated from zinc oxide (ZnO) and aluminium oxide (Al2O-3) has increased significantly. These nanoparticles are widely used as ingredient in cosmetics, and also in food packaging industry although their toxicity status is yet to be studied. Here, we aimed to explore the effect of zinc oxide nanoparticles (ZnO-NPs) and aluminium oxide nanoparticles (ANPs) in human HT29 colon cancer cell line.

Methods: In this study, ZnO-NPs were synthesized by chemical method and ANPs synthesized by sol-gel method and were characterized using UV-Vis spectroscopy, X ray diffraction and Transmittance electron microscopy. The effects of ZnO-NPs and ANPs was determined by cell viability, membrane integrity and colony formation potentials.

Results: ZnO-NPs and ANPs inhibit HT29, colon cancer cell proliferation in a dose dependent manner, and affect the membrane potentials and also prevent the colony formation.

Conclusions: The results suggest that ZnO NPs are found to be more effective than ANPs in reducing colon cancer cell proliferation.

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Conflict of interest statement

Each author declares that he or she has no commercial associations (e.g. consultancies, stock ownership, equity interest, patent/licensing arrangement etc.) that might pose a conflict of interest in connection with the submitted article

Figures

None
Graphical Abstract
Figure 1.
Figure 1.
UV vis spectrum of nanoparticles (a) UV vis spectrum of ANPs (b) ZnO NPs
Figure 2.
Figure 2.
X- ray diffraction pattern of nanoparticles (a) ZnO NPs (b) ANPs
Figure 3.
Figure 3.
Transmission electron microscopic images (a) ZnO NPs (b) ANPs
Figure 4.
Figure 4.
Concentration dependent Cytotoxicity of ZnO NPs and ANPs in HT 29 cells. Cells were exposed to different concentrations of ZnO NPs and ANPs for 48 h and the viability was determined by MTT assay. Unexposed cells as a control and run it in parallel to the exposed groups. Values were the mean±SD from three independent experiments. Significance was indicated by bp<0.001 and cp<0.01 verus control
Figure 5.
Figure 5.
Effect of ZnO NPs and ANPs on LDH leakage in HT 29 cells. Cells were exposed to 50 and 100 μg/mL for 48 h. Control cells cultured in nanoparticles free medium run in parallel to the exposed groups. Values were the mean±SD from three independent experiments. Significance was indicated by cp<0.01 verus control
Figure 6.
Figure 6.
Effect of ZnO NPs and ANPs on MDA level in HT 29 cells. Cells were exposed to 50 and 100 μg/mL for 48 h. Control cells cultured in nanoparticles free medium run in parallel to the exposed groups. Values were the mean±SD from three independent experiments. Significance was indicated by bp<0.001 and cp<0.01 verus control
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