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. 1987 Jun;208(1-2):349-52.
doi: 10.1007/BF00330464.

Plasmid maintenance in Bacillus subtilis recombination-deficient mutants

J C Alonso, J F Viret, R H Tailor

Plasmid maintenance in Bacillus subtilis recombination-deficient mutants

J C Alonso et al. Mol Gen Genet. 1987 Jun.

Abstract

An isogenic set of 11 recombination-deficient mutant strains of Bacillus subtilis has been constructed. Whereas plasmid pUB110 is stably maintained in such Rec- cells, the high copy number plasmid pC194 is unstable. Instability in Rec- strains could be mostly attributed to the deleterious effect of the presence of the plasmid on the Rec- cells' growth capability. In part, instability of pC194 derivatives could also be correlated with the presence of an unusually high amount of multimeric DNA molecules.

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