doi: 10.1007/978-1-4939-9488-5_5.
Analyses of Cholesterol Metabolites of Optic Nerve Using GC-MS Methods
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- PMID: 31127546
- DOI: 10.1007/978-1-4939-9488-5_5
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Analyses of Cholesterol Metabolites of Optic Nerve Using GC-MS Methods
Methods Mol Biol.
2019.
Abstract
Gas chromatography-mass spectrometry (GC-MS) is considered the gold standard for analyzing and quantifying the presence of biological compounds in tissue samples due to its high sensitivity, peak resolution, and reproducibility. In this chapter, we describe a step-by-step modified Bligh and Dyer protocol for lipid extraction from the optic nerve tissue and a procedure for GC-MS analyses of the lipid extract. These protocols are based on our experience and can be modified depending on samples and compounds of interest.
Keywords: Cholesterol; Gas chromatography-mass spectrometry (GC-MS); Lipid extraction; Lipidomics; Lipids.
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