Olsenella faecalis sp. nov., an anaerobic actinobacterium isolated from human faeces

Abstract

A novel actinobacterial strain, designated KGMB04489^T, was isolated from the faeces of a healthy Korean. Cells of the strain were strictly anaerobic, Gram-stain-positive and short-rod-shaped. On the basis of 16S rRNA gene sequence similarity, strain KGMB04489^T belonged to the genus Olsenella and was most closely related to Olsenella scatoligenes SK9K4^T (94.3 %), Olsenella uli ATCC 49627^T (93.5 %), Olsenella umbonata lac31^T (93.4 %) and Olsenella profusa D315A-29^T (93.3 %). The major end product was lactic acid. The DNA G+C content was 65.5 mol%. The major cellular fatty acids of strain KGMB04489^T were C18 : 1cis9, C18 : 1cis9 DMA and C16 : 0. Strain KGMB04489^T contained meso-diaminopimelic acid as the diamino acid in the peptidoglycan. The polar lipids consisted of an unidentified phospholipid, six unidentified glycolipids and an unidentified lipid. Based on phylogenetic, physiological and chemotaxonomic characteristics, strain KGMB04489^T is considered to represent a novel species within the genus Olsenella, for which the name Olsenellafaecalis sp. nov. is proposed. The type strain is KGMB04489^T (=KCTC 15699^T=CCUG 72345^T).

Keywords: Olsenella faecalis; actinobacteria; human faeces; taxonomy.