In vitro/in vivo hepatoprotective properties of 1-O-(4-hydroxymethylphenyl)-α-L-rhamnopyranoside from Moringa oleifera seeds against carbon tetrachloride-induced hepatic injury

Abstract

1-O-(4-hydroxymethylphenyl)-α-L-rhamnopyranoside (MPG) is a phenolic glycoside that exists in Moringa oleifera seeds with various health benefits, whereas its hepatoprotective effect is lacking clarification. Herein, MPG was isolated from Moringa oleifera seeds, and its hepatoprotection against CCl₄-induced hepatotoxicity in L02 cells and ICR mice was investigated. Toxicity studies showed that MPG did not induce significant changes in organ coefficients and histological analysis, as well as exhibited no cytotoxicity. In vitro studies indicated that MPG substantially increased cell viability and intracellular SOD activities, and significantly inhibited LDH leakage in CCl₄-treated cells. In vivo studies demonstrated that MPG significantly alleviated CCl₄-induced hepatotoxicity in mice, as indicated by diagnostic indicators of hepatic injury, as well as the histopathological analysis. Moreover, MPG reduced the lipid peroxidation levels and regulated the inflammatory cytokines. Notably, MPG substantially suppressed the significant elevation of ROS production in hepatocytes of mice intoxicated with CCl₄. Moreover, TUNEL assay demonstrated that MPG obviously inhibited hepatic apoptosis induced by CCl₄. Altogether, these results suggested that MPG has excellent liver-protecting effects against hepatocytotoxicity induced by CCl₄ in mice and L02 cells, which can be further developed as a valuable functional food additive or drug for the treatment of hepatic injury.

Keywords: 1-O-(4-hydroxymethylphenyl)-α-L-rhamnopyranoside; Acute toxicity; Carbon tetrachloride; Hepatotoxicity; Moringa oleifera seeds; Oxidative stress.