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. 2019 May 28;9(1):7960.
doi: 10.1038/s41598-019-44478-8.

Enhanced osteogenic proliferation and differentiation of human adipose-derived stem cells on a porous n-HA/PGS-M composite scaffold

Yaozong Wang  1   2 , Naikun Sun  3 , Yinlong Zhang  4 , Bin Zhao  4 , Zheyi Zhang  5 , Xu Zhou  6 , Yuanyuan Zhou  4 , Hongyi Liu  4 , Ying Zhang  2 , Jianguo Liu  7
Affiliations

Enhanced osteogenic proliferation and differentiation of human adipose-derived stem cells on a porous n-HA/PGS-M composite scaffold

Yaozong Wang et al. Sci Rep. .

Abstract

This study explored the applicability, cellular efficacy, and osteogenic activities of porous nano-hydroxyapatite/Poly (glycerol sebacate)-grafted maleic anhydride (n-HA/PGS-g-M) composite scaffolds. Nuclear magnetic resonance (NMR) analyses indicated that approximately 43% of the hydroxide radicals in PGS were displaced by maleic anhydride. Resonance bands at 1036 cm-1 occurred in scaffolds containing nHA powders, and peak areas increased when n-HA weight increased in PGS-M-n-HA-0.4, PGS-M-n-HA-0.5, and PGS-M-n-HA-0.6 scaffolds. The n-HA/PGS-g-M composite scaffolds exhibited porous microstructure with average pore size of 150-300 µm in scanning electron microscopy (SEM) analysis. Differential scanning calorimetry (DSC) identified the glass transition temperature (Tg) as -25-30 °C, indicative of quality resilience. The modulus of compressibility increased when n-HA content increased. Interestingly, viability of human adipose-derived stem cells (hADSCs) in vitro and expression of the osteogenic related genes RUNX2, OCN, and COL1A1 was enhanced in the n-HA/PGS-g-M composite scaffolds compared to those factors observed in PGS-g-M scaffolds. Finally, simulated body fluid (SBF) tests indicated more apatite deposits on the surface of n-HA/PGS-g-M scaffolds compared to PGS-g-M scaffolds. Overall, porous n-HA/PGS-g-M composite scaffolds possessed acceptable biocompatibility and mechanical properties, and they stimulated hADSC cell proliferation and differentiation. Given these qualities, the composite scaffolds have potential applications in bone tissue engineering.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Chemical characterizations of PGS-g-M scaffolds and PGS-M-n-HA-0.4, PGS-M-n-HA-0.5, and PGS-M-n-HA-0.6 composite scaffolds. (A) The 1H-NMR spectrogram of PGS-g-M. (B) The FT-IR spectrogram of n-HA powder, PGS-g-M scaffolds, and PGS-M-n-HA-0.4, PGS-M-n-HA-0.5, and PGS-M-n-HA-0.6 composite scaffolds.
Figure 2
Figure 2
SEM images of the PGS-g-M scaffolds (A) and the PGS-M-n-HA-0.4 (B), PGS-M-n-HA-0.5 (C), and PGS-M-n-HA-0.6 composite scaffolds (D).
Figure 3
Figure 3
Physical characterizations of PGS-g-M scaffolds and PGS-M-n-HA-0.4, PGS-M-n-HA-0.5, and PGS-M-n-HA-0.6 composite scaffolds. (A) Differential scanning calorimetry (DSC) curves; (B) thermogravimetric analysis (TGA) curves; (C) the stress-strain curve; (D) the modulus of compressibility graphs.
Figure 4
Figure 4
The biomedical applications of PGS-M-n-HA-0.4, PGS-M-n-HA-0.5, and PGS-M-n-HA-0.6 composite scaffolds in bone regeneration in vitro. (A) the effects of these scaffolds on cell proliferation in human adipose-derived stem cells (hADSCs); (B) the expression levels of proinflammatory factor IL-6, and the expression levels of genes associated with osteoblast differentiation by qRT-PCR; The levels of RUNX2, OCN proteins by western blot (C) and immunohistochemical staining (D).
Figure 5
Figure 5
SEM images of scaffolds in simulated body fluid (SBF) from 1 week to 4 weeks. The upper left corner: magnification of SEM images.
Figure 6
Figure 6
The reaction scheme for the synthesis of PGS-g-M. PGS-g-M, poly (glycerol sebacate)-graft-Maleic anhydride.
See this image and copyright information in PMC

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References

    1. Betz RR. Limitations of autograft and allograft: new synthetic solutions. Orthopedics. 2002;25:561–570. - PubMed
    1. Pobloth AM, et al. Mechanobiologically optimized 3D titanium-mesh scaffolds enhance bone regeneration in critical segmental defects in sheep. Science Translational Medicine. 2018;10:eaam8828. doi: 10.1126/scitranslmed.aam8828. - DOI - PubMed
    1. Omar O, Dahlin A, Gasser A, Dahlin C. Tissue dynamics and regenerative outcome in two resorbable non‐cross‐linked collagen membranes for guided bone regeneration: A preclinical molecular and histological study in vivo. Clinical oral implants research. 2018;29:7–19. doi: 10.1111/clr.13032. - DOI - PubMed
    1. Ando K, et al. Effects of a self-assembling peptide as a scaffold on bone formation in a defect. PloS one. 2018;13:e0190833. doi: 10.1371/journal.pone.0190833. - DOI - PMC - PubMed
    1. Yoshimoto H, Shin Y, Terai H, Vacanti J. A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering. Biomaterials. 2003;24:2077–2082. doi: 10.1016/S0142-9612(02)00635-X. - DOI - PubMed

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