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. 2019 May;113(3):133-142.
doi: 10.1080/20477724.2019.1624076. Epub 2019 May 30.

Dynamics of Plasmodium falciparum genetic diversity among asymptomatic and symptomatic children in three epidemiological areas in Cote d'Ivoire

Akpa Paterne Gnagne  1   2 , Abibatou Konate  1   3 , Akoua Valérie Bedia-Tanoh  1   3 , Mireille Amiah-Droh  1   2 , Hervé Ignace Eby Menan  3 , Assanvo Simon-Pierre N'Guetta  2 , William Yavo  1   3
Affiliations

Dynamics of Plasmodium falciparum genetic diversity among asymptomatic and symptomatic children in three epidemiological areas in Cote d'Ivoire

Akpa Paterne Gnagne et al. Pathog Glob Health. 2019 May.

Abstract

Asymptomatic carriers of Plasmodium are considered a reservoir of the parasite in humans. Therefore, in order to be effective, new malaria elimination strategies must take these targets into account. The aim of this study was to analyse genetic diversity of Plasmodium falciparum among schoolchildren in three epidemiological areas in Côte d'Ivoire. This was a cross-sectional study carried out from May 2015 to April 2016 in a primary school in rural and urban areas of San Pedro, Grand-Bassam and Abengourou, during the rainy season and the dry season. A total of 282 Plasmodium falciparum isolates were genotyped using Nested PCR of Pfmsp1 and Pfmsp2 genes. The overall frequency of K1, Mad20 and RO33 alleles was 81.6%, 53.4% and 57% for Pfmsp1 respectively. For Pfmsp2, this frequency was 84.3% and 72.2% for 3D7 and FC27. K1, Mad20 and FC27 Frequencies were significantly higher in Abengourou compared to other sites. Overall, the frequency of MIs was significantly higher in Abengourou for Pfmsp1 and Pfmsp2. However, Mad20 and RO33 alleles were significantly higher in the rainy season. No significant difference was observed between Pfmsp2 alleles in both seasons. Frequency of the 3D7 allele was significantly higher in symptomatic patients. MIs and COI increased with parasitemia for Pfmsp1and Pfmsp2. The data can be added to that available for monitoring and control of P. falciparum malaria. Further studies combining the entomological inoculation rate and the genetic diversity of P. falciparum will allow us to shed light on our understanding of the epidemiology of this parasite.

Keywords: Côte d’Ivoire; asymptomatic carrier; genetic diversity.

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Figures

Figure 1.
Figure 1.
Study sites (CNTIG, 2012; Field surveys, 2017).
See this image and copyright information in PMC

References

    1. OMS , 2018. World malaria report 2018.
    1. Koffi D, Varela M-L, Loucoubar C, et al. Longitudinal analysis of antibody responses in symptomatic malaria cases do not mirror parasite transmission in peri-urban area of cote d’Ivoire between 2010 and 2013. PloS One. 2017;12:e0172899. - PMC - PubMed
    1. Yavo W, Konaté A, Mawili-Mboumba DP, et al. Genetic Polymorphism of msp1 and msp2 in Plasmodium falciparum Isolates from Côte d’Ivoire versus Gabon. J Parasitol Res. 2016;2016:3074803. - PMC - PubMed
    1. Gardner MJ, Shallom SJ, Carlton JM, et al. Sequence of Plasmodium falciparum chromosomes 2, 10, 11 and 14. Nature. 2002;419:531–534. - PubMed
    1. Gunawardena S, Karunaweera ND.. Advances in genetics and genomics: use and limitations in achieving malaria elimination goals. Pathog Glob Health. 2015;109:123–141. - PMC - PubMed

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