Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Feb;24(2):777-784.
doi: 10.1007/s00784-019-02971-4. Epub 2019 Jun 1.

Quaternary ammonium compound as antimicrobial agent in resin-based sealants

Isadora Martini Garcia  1 , Stéfani Becker Rodrigues  1 , Gabriela de Souza Balbinot  1 , Fernanda Visioli  2 , Vicente Castelo Branco Leitune  1 , Fabrício Mezzomo Collares  3
Affiliations

Quaternary ammonium compound as antimicrobial agent in resin-based sealants

Isadora Martini Garcia et al. Clin Oral Investig. 2020 Feb.

Abstract

Objectives: The aim of this study was to evaluate the influence of [2-(methacryloyloxy)ethyl] trimethylammonium chloride (METAC) in the physico-chemical properties, antibacterial activity and cytotoxicity of an experimental resin-based sealant.

Materials and methods: An experimental resin-based sealant was formulated with dimethacrylates and a photoinitiator system. METAC was added at 2.5 wt.% (G2.5%) and 5 wt.% (G5%) into the experimental resin-based sealant, and one group remained without METAC as control (GCTRL). The resin-based sealants were analysed for polymerization behaviour and degree of conversion (DC), Knoop hardness (KHN) and softening in solvent (ΔKHN), ultimate tensile strength (UTS), contact angle, surface free energy (SFE), immediate and long-term micro-shear bond strength (μ-SBS) and antibacterial activity and cytotoxicity against human keratinocytes.

Results: The experimental resin-based sealants presented different polymerization behaviours without significant differences in the DC (p > 0.05). There was no significant difference for initial KHN (p > 0.05). The ΔKHN ranged from 51.62 (±3.70)% to 62.40 (±4.14)%, with higher values for G5% (p < 0.05). G2.5% and G5% had decreased μ-SBS between immediate and long-term tests (p < 0.05) without significant differences among groups in the immediate and long-term analyses (p > 0.05). There were no significant differences for UTS, contact angle and SFE among groups (p > 0.05). G2.5% and G5% presented immediate and long-term antibacterial activity (p < 0.05) without cytotoxicity compared to GCTRL (p > 0.05).

Conclusion: The addition of METAC provided antibacterial activity to the experimental resin-based sealant.

Clinical relevance: METAC is an effective quaternary ammonium compound as an antibacterial agent for resin-based sealants without cytotoxic effects against human keratinocytes.

Keywords: Antibacterial agents; Dental caries; Pit and fissure sealants; Polymerization; Quaternary ammonium compound.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Braz Oral Res. 2017 May 04;31:e31 - PubMed
    1. J Dent. 2018 Jun;73:57-60 - PubMed
    1. J Dent. 2017 Jul;62:85-90 - PubMed
    1. J Dent Res. 2017 Jul;96(7):754-761 - PubMed
    1. Oral Health Prev Dent. 2017;15(1):79-87 - PubMed

LinkOut - more resources