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. 2019 May 15:10:301.
doi: 10.3389/fendo.2019.00301. eCollection 2019.

Competing Endogenous RNA Network Analysis Reveals Pivotal ceRNAs in Adrenocortical Carcinoma

Weiwei Liang  1 , Fangfang Sun  2
Affiliations

Competing Endogenous RNA Network Analysis Reveals Pivotal ceRNAs in Adrenocortical Carcinoma

Weiwei Liang et al. Front Endocrinol (Lausanne). .

Abstract

Objective: To construct ceRNA network and identify pivotal competing endogenous RNAs (ceRNAs) in adrenocortical carcinoma (ACC) using ceRNA network analysis. Methods: The RNA sequencing expression data of 77 ACCs in TCGA were obtained from GEPIA. Cancer specific ceRNAs, cancer specific microRNAs (miRNAs), and cancer specific messenger RNAs (mRNAs) were identified. The interaction of cancer specific miRNAs with cancer specific ceRNAs and cancer specific mRNAs were predicted. CeRNA network was constructed and visualized by Cytoscape 3.7.0 software. The genes in ceRNA network regulated GO terms and regulated pathways were performed by function analysis. Survival analysis of pivotal ceRNAs was performed for the pivotal lncRNAs. Result: Twenty-eight cancer specific ceRNAs, 149 cancer specific miRNAs, and 104 mRNAs were identified. CeRNA network was constructed including 10 ceRNAs, 35 miRNAs, and 34 mRNAs. The genes in ceRNA network regulated GO terms and were classified into three groups: cellular component (CC), molecular function (MF), and biological process (BP). The genes in ceRNA network regulated the following pathways: leukocyte transendothelial migration, and proteoglycans in cancer. Survival analysis showed that CTB-63M22.1 and RP1-241P17.4 were significantly associated with ACC patient disease free survival and overall survival. Conclusion: This study has constructed ceRNA networks in ACC. The study provides a set of pivotal ceRNAs for future investigation into the molecular mechanisms.

Keywords: adrenocortical carcinoma; ceRNA; ceRNA network analysis; lncRNA; non-coding RNA.

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Figures

Figure 1
Figure 1
(A) ceRNA network in ACC. Diamond in yellow represented cancer specific ceRNA. Ellipse in gray represented cancer specific mRNA. Rectangle in blue represented cancer specific mRNA. (B) Down-regulated ceRNAs regulated ceRNA network. (C) Up-regulated ceRNAs regulated ceRNA network.
Figure 2
Figure 2
Gene ontology analysis for biological process of genes involved in the ceRNA network in ACC.
Figure 3
Figure 3
Gene ontology analysis for cellular component of genes involved in the ceRNA network in ACC.
Figure 4
Figure 4
Gene ontology analysis for molecular function of genes involved in the ceRNA network in ACC.
Figure 5
Figure 5
(A–J): Overall survival plot of 10 pivotal ceRNAs using 77 ACC patient data from TCGA database.
Figure 6
Figure 6
(A–J): Disease free survival plot of 10 pivotal ceRNAs using 77 ACC patient data from TCGA database.
See this image and copyright information in PMC

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