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. 1987 Jun 20;195(4):949-52.
doi: 10.1016/0022-2836(87)90499-2.

Specific destruction of the second lac operator decreases repression of the lac operon in Escherichia coli fivefold

E Eismann  1 , B von Wilcken-Bergmann, B Müller-Hill
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Specific destruction of the second lac operator decreases repression of the lac operon in Escherichia coli fivefold

E Eismann et al. J Mol Biol. .

Abstract

The second operator of the lac operon, located within the 5'-coding region of the lacZ gene, was specifically destroyed by means of oligonucleotide-directed mutagenesis. Eight of its bases were exchanged without altering the wild-type amino acid sequence of beta-galactosidase. The mutation was transferred onto an F'lac+I+O+Z+pro+ episome. We observed a fivefold decrease in repression of beta-galactosidase expression compared to that in the wild-type.

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