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. 2019 Jun 4;14(1):53.
doi: 10.1186/s13000-019-0831-3.

Overexpression of microRNA-423-3p indicates poor prognosis and promotes cell proliferation, migration, and invasion of lung cancer

Rukun Wang  1 , Gaofeng Li  2 , Guoyan Zhuang  3 , Shuying Sun  4 , Zhihui Song  5
Affiliations

Overexpression of microRNA-423-3p indicates poor prognosis and promotes cell proliferation, migration, and invasion of lung cancer

Rukun Wang et al. Diagn Pathol. .

Abstract

Background: Lung cancer is one of the common malignant tumors worldwide with high incidence and mortality. MicroRNA-423-3p (miR-423-3p) acts as an oncogene in several types of cancers. The aim of this study is to reveal the clinical significance and biological function of miR-423-3p in lung cancer.

Methods: The expression of miR-423-3p was detected in lung cancer specimens by reverse transcription-quantitative polymerase chain reaction (qRT-PCR) assay. Kaplan-Meier survival and Cox regression analyses were used to investigate the prognostic significance of miR-423-3p in lung cancer. CCK-8 and Transwell assays were used to determine the functional role of miR-423-3p in lung cancer.

Results: We observed that miR-423-3p was significantly upregulated in lung cancer tissues and cell lines. Overexpression of miR-423-3p was significantly associated with lymph node metastasis, TNM stage, and poor prognosis. Multivariate Cox regression analysis results showed that miR-423-3p was an independent prognostic indicator for lung cancer patients. Results of functional analyses revealed that overexpression of miR-423-3p promoted cell proliferation, migration, and invasion in lung cancer cells.

Conclusions: These results indicated that miR-423-3p acts as an oncogene and promotes cell proliferation migration, and invasion of lung cancer. And miR-423-3p may serve as a potential prognostic biomarker and therapeutic target for the treatment of lung cancer.

Keywords: Invasion; Lung cancer; Migration; Prognosis; Proliferation; microRNA-423-3p.

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Conflict of interest statement

The authors declare that they had no competing interests.

Figures

Fig. 1
Fig. 1
Expression of miR-423-3p in lung cancer tissues and cell lines. a Expression of miR-423-3p was upregulated in lung cancer tissues compared with normal tissues. (***P < 0.001). b Expression of miR-423-3p was increased in lung cancer cell lines (A549, H1299, HCC827, and A427) compared with normal cell line BEAS-2B. (***P < 0.001)
Fig. 2
Fig. 2
Upregulation of miR-423-3p correlated with poor prognosis of lung cancer patients according to the result of the Kaplan-Meier method
Fig. 3
Fig. 3
Overexpression of miR-423-3p promotes cell proliferation, while inhibition of miR-423-3p suppresses cell proliferation of A549 and H1299 cells. a Expression of miR-423-3p in mimic NC, miR-423-3p mimic, inhibitor NC, or miR-423-3p inhibitor-transfected cells. (**P < 0.01, ***P < 0.001). b Effects of miR-423-3p on lung cancer cell proliferation in A549 and H1299 cells. (*P < 0.05)
Fig. 4
Fig. 4
Overexpression of miR-423-3p promotes cell migration and invasion, while inhibition of miR-423-3p suppresses cell migration and invasion in A549 and H1299 cells. a Effects of miR-423-3p on lung cancer cell migration. (**P < 0.01, ***P < 0.001). b Effects of miR-423-3p on lung cancer invasion. (**P < 0.01, ***P < 0.001)
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