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. 2019 Jul 26;57(8):e00167-19.
doi: 10.1128/JCM.00167-19. Print 2019 Aug.

Real-Time PCR Assay for Differentiation of Typhoidal and Nontyphoidal Salmonella

Satheesh Nair  1 , Vineet Patel  2   3 , Tadgh Hickey  2   3 , Clare Maguire  2 , David R Greig  2 , Winnie Lee  2 , Gauri Godbole  2 , Kathie Grant  2 , Marie Anne Chattaway  2
Affiliations

Real-Time PCR Assay for Differentiation of Typhoidal and Nontyphoidal Salmonella

Satheesh Nair et al. J Clin Microbiol. .

Abstract

Rapid and accurate differentiation of Salmonella spp. causing enteric fever from nontyphoidal Salmonella is essential for clinical management of cases, laboratory risk management, and implementation of public health measures. Current methods used for confirmation of identification, including biochemistry and serotyping as well as whole-genome sequencing analyses, take several days. Here we report the development and evaluation of a real-time PCR assay that can be performed directly on crude DNA extracts from bacterial colonies for the rapid identification of typhoidal and nontyphoidal Salmonella.

Keywords: Nontyphoidal salmonellae; Salmonella; real-time PCR; typhoidal salmonellae; whole-genome sequencing.

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Figures

FIG 1
FIG 1
Selection of representative strains to test in silico. Shown is a population structure of Salmonella received at PHE between 2016 and 2017 and strains tested by PCR in this study, totaling 19,221 strains. Strains are color coded by main eBURST groups (eBG). Representative strains (highlighted in orange) from each sequence type within an eBG containing 3 or more isolates were selected for in silico detection of the seven genes (ttr, sseJ, srfJ, tviB, SPC0869, SPA2308, and staG).
See this image and copyright information in PMC

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