Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Oct;14(10):1715-1716.
doi: 10.4103/1673-5374.257525.

New ex vivo demyelination/remyelination models to defeat multiple sclerosis and neuromyelitis optica

Yiting Liu  1
Affiliations

New ex vivo demyelination/remyelination models to defeat multiple sclerosis and neuromyelitis optica

Yiting Liu. Neural Regen Res. 2019 Oct.
No abstract available

PubMed Disclaimer

Conflict of interest statement

None

Figures

Figure 1
Figure 1
Distinct glia responses in MS and NMO rAb-slice models during damage and recovery. Cerebellar slice cultures are prepared from mice at postnatal day 10 and cultured for 7–10 days prior to treatment. Slices are treated with MS myelin-specific rAb or NMO aquaporin-4 + rAb (human IgG1) at 20 µg/mL in the presence of 10% (vol/vol) normal human serum as a source of complement for 24–48 hours to induce damage. Then the treatment is removed and slices are cultured in medium for additional 7–14 days for recovery. Distinct patterns of rAb-induced complement-dependent cytotoxicity contribute to demyelination injury. Different glial responses are coupled with disparate capacities for remyelination after treatment withdrawal. MS: Multiple sclerosis; NMO: neuromyelitis optica; N: neuron; AST: astrocytes; OL: oligodendrocyte; MG: microglia; rAb: recombinant antibody.
See this image and copyright information in PMC

References

    1. Bennett JL, Lam C, Kalluri SR, Saikali P, Bautista K, Dupree C, Glogowska M, Case D, Antel JP, Owens GP, Gilden D, Nessler S, Stadelmann C, Hemmer B. Intrathecal pathogenic anti-aquaporin-4 antibodies in early neuromyelitis optica. Ann Neurol. 2009;66:617–629. - PMC - PubMed
    1. Krumbholz M, Meinl E. B cells in MS and NMO: pathogenesis and therapy. Semin Immunopathol. 2014;36:339–350. - PubMed
    1. Liu Y, Given KS, Owens GP, Macklin WB, Bennett JL. Distinct patterns of glia repair and remyelination in antibody-mediated demyelination models of multiple sclerosis and neuromyelitis optica. Glia. 2018;66:2575–2588. - PMC - PubMed
    1. Liu Y, Given KS, Harlow DE, Matschulat AM, Macklin WB, Bennett JL, Owens GP. Myelin-specific multiple sclerosis antibodies cause complement-dependent oligodendrocyte loss and demyelination. Acta Neuropathol Commun. 2017;5:25. - PMC - PubMed
    1. Lloyd AF, Davies CL, Miron VE. Microglia: origins, homeostasis, and roles in myelin repair. Curr Opin Neurobiol. 2017;47:113–120. - PubMed