Analysis of Bone Mineral Profile After Prolonged Every-Other-Day Feeding in C57BL/6J Male and Female Mice

Abstract

Intermitted fasting or every-other-day feeding (EOD) has many positive effects in rodents and humans. Our goal was to describe how EOD influences bone mineral composition in female and male mice under prolonged EOD feeding. Male and female adult mice were fed EOD for 9 months. After this time, we used a direct method of measurement of mineral components in ashes of long bones (humerus and radius) to estimate the content of calcium (Ca), phosphorus (P), potassium (K), magnesium (Mg), and sodium (Na). We also performed histological analysis of sections of long bones. We found no significant changes in mineral composition between ad libitum and EOD fed males and females. We noted higher Ca and P contents in control males vs. females and lower content of Mg in control males vs. females. We observed the presence of marrow adipose tissue (MAT) in sections of EOD-fed females. EOD without supplementation during feeding days did not increase loss of mineral content of bones in C57BL/6J mice, but the presence of MAT only in EOD females indicates a gender-dependent response to EOD treatment in C57BL/6J mice.

Keywords: Bone mineral profile; Caloric restriction; Every-other-day feeding; Gender difference.

Fig. 1

Morphology of bones (H&E) C57BL/6J mice: males and females. AL, ad libitum fed; EOD, every-other-day-fed animals; objective magnification × 10, scale bar 100 μm; insert in EOD female panel indicates presence of MAT (marrow adipose tissue)—black arrow; green arrows—macrophages containing hemosiderin; yellow asterisk—collagen fibers; M,- megacariocytes