Persistence of fecal indicator bacteria associated with zooplankton in a tropical estuary-west coast of India

Abstract

In a study carried out during 2014, bacteria associated with zooplankton in the Zuari estuary were three to four orders of magnitude higher in abundance than in seawater. The live zooplankton carried much more bacterial load compared with the carcasses, and the fecal pellets harbored the highest density of bacteria, i.e., 8 × 10¹³ CFU cm^-3. The diversity of bacteria was higher in live zooplankton and also in seawater. But the activity of the zooplankton-associated bacteria was much higher compared with the free-living ones. Most of the associated bacteria belonged to the genus Enterobacter, Pseudomonas, Aeromonas, and Bacillus. In growth experiments, Aeromonas and Bacillus were found to have lower salinity optima than Enterobacter (20 psu) and Vibrio and Pseudomonas (normal seawater salinity). Better growth of bacteria was observed in the medium containing the diatom Chaetoceros sp. than Navicula sp. Bacterial isolates were also able to survive in oligotrophic conditions and produce optimum biomass in 2 days at salinity 5 psu, but in freshwater, the bacteria took a week's time to attain the optima. At salinities 0-35, the bacteria survived even for 3 months without nutrient addition, indicating resilience in these bacteria and mechanisms to persist in the estuaries even in adverse conditions.

Keywords: Aeromonas; Associated bacteria; Enterobacteria; Zooplankton; Zuari estuary.