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Case Reports
. 2019 Jun 11;3(11):1774-1777.
doi: 10.1182/bloodadvances.2019000121.

Adoptive T-cell therapy for pediatric cytomegalovirus-associated retinitis

Shiney Seo  1 , Corey Smith  2 , Christopher Fraser  3 , Rajan Patheja  1 , Shaheen P Shah  1   4 , Sweera Rehan  5 , Pauline Crooks  5 , Michelle A Neller  5 , Rajiv Khanna  5
Affiliations
Case Reports

Adoptive T-cell therapy for pediatric cytomegalovirus-associated retinitis

Shiney Seo et al. Blood Adv. .

Abstract

  1. TCRαβ+/CD19+-depleted haploidentical HSCT was used to restore immunity in a pediatric patient with combined immunodeficiency syndrome.

  2. Posttransplant drug-resistant CMV retinitis was successfully treated with T cells expanded from a haploidentical HSCT donor.

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Conflict of interest statement

Conflict-of-interest disclosure: C.S. and R.K. receive research and consultancy funding from Atara Biotherapeutics Inc. R.K. is also appointed as an advisor on the Atara Biotherapeutics Scientific Advisory Board. C.S. and R.K. hold international patents or patent applications that cover CMV epitope sequences and their use in adoptive immunotherapy. The remaining authors declare no competing financial interests.

Figures

None
Graphical abstract
Figure 1.
Figure 1.
Cellular immunotherapy for CMV-associated retinitis. (A) CMV viral titer during the course of treatment overlaid with antiviral regimens and times for detection of retinitis. Dotted lines represent the times of T-cell infusion. (B) Flow cytometric analysis showing the characteristics of the donor-derived CMV-specific T-cell therapy. The production of IFN-γ was determined using a standard intracellular cytokine assay. (C) The top left panel represents fundus photography showing severe active retinitis and periphlebitis in the right eye inferotemporal quadrant (pretherapy). Progressive resolution of retinitis is shown after 1, 2, and 3 CMV-specific T-cell infusions. The right hand panels represent wide-field fundus photographs showing significantly improved retinitis after 6 T-cell infusions. (D) Immunological reconstitution after ACT was assessed using the QuantiFERON-CMV assay. Data represent IFN-γ (IU/mL) titers after stimulation with either the CMV peptides or mitogen and subtracting the no-antigen control. Dotted lines represent the times of T-cell infusions.
See this image and copyright information in PMC

References

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