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. 2019 Aug;411(20):5175-5186.
doi: 10.1007/s00216-019-01897-w. Epub 2019 Jun 11.

An ultrasensitive electrochemiluminescence immunosensor for alpha-fetoprotein based on a poly(aniline-luminol)/graphene oxide nanocomposite

Lu Wei  1 , Yanhui Zhang  1 , Nurguzal Eziz  2 , Yaru Yang  1 , Guixin Li  3 , Ming Guan  1
Affiliations

An ultrasensitive electrochemiluminescence immunosensor for alpha-fetoprotein based on a poly(aniline-luminol)/graphene oxide nanocomposite

Lu Wei et al. Anal Bioanal Chem. 2019 Aug.

Abstract

An ultrasensitive electrochemiluminescence (ECL) immunosensor for alpha-fetoprotein (AFP) detection based on a poly(aniline-luminol)/graphene oxide nanocomposite was developed. The nanocomposite, which was prepared using a fast and simple chemical oxidation strategy for the first time, showed excellent ECL performance. This outstanding ECL performance is due to the formation of poly(aniline-luminol) on the graphene oxide (GO) surface and the excellent electron-transfer properties of GO. Moreover, the poly(aniline-luminol)/graphene oxide nanocomposite has abundant amino groups at its surface, making it a good platform for biomacromolecule labeling. Using the nanocomposite, a novel ECL immunosensor for the determination of AFP was successfully developed. Anti-AFP was immobilized on the surface of a poly(aniline-luminol)/graphene oxide nanocomposite-modified electrode using a glutaraldehyde crosslinking method to form the ECL immunosensor. The AFP was then captured at the modified electrode surface through an antigen-antibody immunoreaction. When the AFP was captured by its antibody, the ECL intensity decreased. This ECL immunosensor for the detection of AFP exhibited a linear range of 1.7 × 10-12 to 1.7 × 10-8 mg mL-1 and a detection limit of 5 × 10-13 mg mL-1, indicating high sensitivity and linearity across a wide concentration range. Furthermore, the immunosensor was successfully applied to determine AFP in a real-world human serum sample. Graphical abstract A new poly(aniline-luminol)/graphene oxide nanocomposite was prepared using a fast and simple strategy for the first time, and an ultrasensitive electrochemiluminescence (ECL) immunosensor for alpha-fetoprotein (AFP) detection based on this nanocomposite was developed.

Keywords: Alpha-fetoprotein; Chemical oxidation; Electrochemiluminescence; Immunosensor; Poly(aniline-luminol)/graphene oxide nanocomposite.

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