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Review
. 2019:74:e698.
doi: 10.6061/clinics/2019/e698. Epub 2019 Jun 6.

Screening of Strongyloides infection using an ELISA test in transplant candidates

Beatriz Toledo  1 , Marcelo A Corral  1 , Dirce Mary C L Meisel  1 , Maiara Gottardi  1 , Edson Abdala  2 , Silvia F Costa  2 , Lígia Camera Pierrotti  2 , Susana A Z Lescano  1   3 , Elenice M N Gonçalves  4 , Vera L P Castilho  4 , Pedro P Chieffi  5 , Ronaldo C B Gryschek  1   3 , Fabiana M Paula  1   3
Affiliations
Review

Screening of Strongyloides infection using an ELISA test in transplant candidates

Beatriz Toledo et al. Clinics (Sao Paulo). 2019.

Abstract

Objectives: Hyperinfection or disseminated strongyloidiasis has been frequently reported after transplants and is related to high mortality. This study aimed to screen for strongyloidiasis using serological diagnoses in transplant candidates.

Methods: An ELISA test was performed with filariform larvae of Strongyloides venezuelensis as a source of antigen.

Results: In the serum from transplant candidates, anti-Strongyloides IgG antibodies were detected in 35/150 (23.3%) samples by soluble fractions in phosphate buffered saline (PBS), 31/150 (20.7%) samples by soluble fractions in Tris-HCl, 27/150 (18.0%) samples by membrane fractions in PBS and 22/150 (14.7%) samples by membrane fractions in Tris-HCl.

Conclusions: The present results suggest the ELISA test, ideally using soluble fractions of filariform larvae S. venezuelensis in PBS, as an additional strategy for the diagnosis of strongyloidiasis in transplant candidates.

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Conflict of interest statement

No potential conflict of interest was reported.

Figures

Figure 1
Figure 1
Specific anti-Strongyloides IgG expressed as the ELISA index (EI) for serum samples from transplant candidates using S. venezuelensis antigenic fractions (SS, TS, SM and TM). The dashed lines represent the positivity threshold (EI >1.0).
See this image and copyright information in PMC

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References

    1. Olsen A, van Lieshout L, Marti H, Polderman T, Polman K, Steinmann P, et al. Strongyloidiasis - the most neglected of the neglected tropical diseases? Trans R Soc Trop Med Hyg. 2009;103((10)):967–72. doi: 10.1016/j.trstmh.2009.02.013. - DOI - PubMed
    1. Keiser PB, Nutman TB. Strongyloides stercoralis in the Immunocompromised Population. Clin Microbiol Rev. 2004;17((1)):208–17. doi: 10.1128/CMR.17.1.208-217.2004. - DOI - PMC - PubMed
    1. Vilela EG, Clemente WT, Mira RR, Torres HO, Veloso LF, Fonseca LP, et al. Strongyloides stercoralis hyperinfection syndrome after liver transplantation: case report and literature review. Transpl Infect Dis. 2009;11((2)):132–6. doi: 10.1111/j.1399-3062.2008.00350.x. - DOI - PubMed
    1. Khuroo MS. Hyperinfection strongyloidiasis in renal transplant recipients. BMJ Case Rep. 2014;2014:bcr2014205068. doi: 10.1136/bcr-2014-205068. pii. - DOI - PMC - PubMed
    1. Izquierdo I, Briones J, Lluch R, Arqueros C, Martino R. Fatal Strongyloides hyperinfection complicating a gram-negative sepsis after allogeneic stem cell transplantation: a case report and review of the literature. Case Rep Hematol. 2013;2013:860976. doi: 10.1155/2013/860976. - DOI - PMC - PubMed

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