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Review
. 2019 Jul;123(1):4-13.
doi: 10.1038/s41437-019-0209-z. Epub 2019 Jun 12.

Using genetics to understand biology

Paul Nurse  1 , Jacqueline Hayles  2
Affiliations
Review

Using genetics to understand biology

Paul Nurse et al. Heredity (Edinb). 2019 Jul.
No abstract available

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1. Cell cycle (cdc) mutant.
a A cdcts mutant at the permissive temperature. Cells can grow and divide and do not elongate. b A cdcts mutant at the restrictive temperature is unable to divide but continues to grow and so has an elongated cell phenotype. The cell wall and septum are stained with calcofluor
Fig. 2
Fig. 2. Wee mutants.
a Wild-type cells. b Wee mutant cells that are advanced into mitosis and divide at a small cell size. The cell wall and septum are revealed by dark field microscopy
Fig. 3
Fig. 3. Cells undergoing DNA re-replication in the absence of mitosis.
Cells undergoing repeated rounds of DNA replication in the absence of mitosis produce elongated cells with increased ploidy. The nuclei are stained with the DNA-specific dye DAPI
Fig. 4
Fig. 4. Cloning by complementation.
A cdc2ts mutant is able to grow at the restrictive temperature when cells carry the human CDC2 gene on a plasmid. This gene is able to complement the yeast cdc2ts mutant function and cells can grow and divide to form colonies (white arrow heads). Cells that lose the plasmid are no longer able to divide, but continue to grow and form elongated cells (black arrow heads)
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