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. 2019;12(4):578-583.
doi: 10.14202/vetworld.2019.578-583. Epub 2019 Apr 19.

Molecular detection of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolates of chicken origin from East Java, Indonesia

Meutia Hayati  1   2 , Agustin Indrawati  1 , Ni Luh Putu Ika Mayasari  1 , Istiyaningsih Istiyaningsih  2 , Neneng Atikah  2
Affiliations

Molecular detection of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolates of chicken origin from East Java, Indonesia

Meutia Hayati et al. Vet World. 2019.

Abstract

Background and aim: Klebsiella pneumoniae is one of the respiratory disease agents in human and chicken. This bacterium is treated by antibiotic, but this treatment may trigger antibiotic resistance. Resistance gene in K. pneumoniae may be transferred to other bacteria. One of the known resistance genes is extended-spectrum β-lactamase (ESBL). This research aimed to study K. pneumoniae isolated from chicken farms in East Java, Indonesia, by observing the antibiotic resistance pattern and detect the presence of ESBL coding gene within the isolates.

Materials and methods: A total of 11 K. pneumoniae isolates were collected from 141 chicken cloacal swabs from two regencies in East Java. All isolates were identified using the polymerase chain reaction method. Antimicrobial susceptibility was determined by agar dilution method on identified isolates, which then processed for molecular characterization to detect ESBL coding gene within the K. pneumoniae isolates found.

Results: The result of antibiotic sensitivity test in 11 isolates showed highest antibiotic resistance level toward ampicillin, amoxicillin, and oxytetracycline (100%, 100%, and 90.9%) and still sensitive to gentamicin. Resistance against colistin, doxycycline, ciprofloxacin, and enrofloxacin is varied by 90.9%, 54.5%, 27.3%, and 18.2%, respectively. All isolates of K. pneumoniae were classified as multidrug resistance (MDR) bacteria. Resistance gene analysis revealed the isolates harbored as bla SHV (9.1%), bla TEM (100%), and bla CTX-M (90.9%).

Conclusion: All the bacterial isolates were classified as MDR bacteria and harbored two of the transmissible ESBL genes. The presence of antibiotic resistance genes in bacteria has the potential to spread its resistance properties.

Keywords: Klebsiella pneumoniae; antibiotic resistance; chicken; extended-spectrum β-lactamase.

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Figures

Figure-1:
Figure-1:
Amplification of blaSHV gene on Klebsiella pneumoniae. A total of one isolate (B31b) showed a positive result of blaSHV. NTC: Non template control.
Figure-2:
Figure-2:
Amplification of blaTEM gene on Klebsiella pneumoniae. All isolates showed positive results of blaTEM. NTC: Non template control.
Figure-3:
Figure-3:
Amplification of blaCTX-M gene on Klebsiella pneumoniae. A total of 10 isolates showed positive results of blaCTX-M. NTC: Non template control.
See this image and copyright information in PMC

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