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. 2019 May 17:12:3859-3868.
doi: 10.2147/OTT.S193616. eCollection 2019.

Proteomic analysis of cerebrospinal fluid in pediatric acute lymphoblastic leukemia patients: a pilot study

Linghong Guo  1   2 , Honghong Ren  2 , Hao Zeng  2 , Yanqiu Gong  3 , Xuelei Ma  1
Affiliations

Proteomic analysis of cerebrospinal fluid in pediatric acute lymphoblastic leukemia patients: a pilot study

Linghong Guo et al. Onco Targets Ther. .

Abstract

Purpose: Involvement of central nervous system in acute lymphoblastic leukemia (CNSL) remains one of the major causes of pediatric acute lymphoblastic leukemia (ALL) treatment failure. However, the current understanding of the pathological process of CNSL is still limited. This study aimed to better understand the protein expression in cerebrospinal fluid (CSF) of ALL and discover valuable prognostic biomarkers.

Materials and methods: CSF samples were obtained from ALL patients and healthy controls. Comparative proteomic profiling using label-free liquid chromatography-tandem mass spectrometry was performed to detect differentially expressed proteins.

Results: In the present study, 51 differentially expressed proteins were found. Among them, two core clusters including ten proteins (TIMP1, LGALS3BP, A2M, FN1, AHSG, HRG, ITIH4, CF I, C2, and C4a) might be crucial for tumorigenesis and progression of ALL and can be potentially valuable indicators of CNSL.

Conclusion: These differentially expressed proteins of ALL children with central nervous system involvement and normal children may work as diagnostic and prognostic factors of ALL patients.

Keywords: ALL; CSF; central nervous system leukemia; mass spectrometry; proteomics.

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Conflict of interest statement

Disclosure The authors report no conflicts of interest in this work.

Figures

Figure 1
Figure 1
Hierarchical clustering of global proteins.
Figure 2
Figure 2
Gene Ontology (GO) and REACTOME pathway analysis of quantified proteins. (A) Top ten biological processes obtained from GO analysis of all differentially expressed proteins. (B) Top ten pathways obtained from REACTOME pathway analysis of all differentially expressed proteins. (C) Biological processes obtained from GO analysis of cluster 1 proteins. (D) Top ten pathways obtained from REACTOME pathway analysis of cluster 1 proteins.
Figure 3
Figure 3
Protein–protein interaction (PPI) and MCODE analysis of differentially expressed proteins. (A) Proteins and interactions of cluster 1 identified by MCODE analysis. (B) Proteins and interactions of cluster 2 identified by MCODE analysis. (C) Proteins and interactions of PPI network.
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