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. 2019 May 21:10:619.
doi: 10.3389/fphys.2019.00619. eCollection 2019.

Comparative Expression Profiling of Wild Type Drosophila Malpighian Tubules and von Hippel-Lindau Haploinsufficient Mutant

Marilena Ignesti  1 , Davide Andrenacci  2   3 , Bettina Fischer  4   5 , Valeria Cavaliere  1 , Giuseppe Gargiulo  1
Affiliations

Comparative Expression Profiling of Wild Type Drosophila Malpighian Tubules and von Hippel-Lindau Haploinsufficient Mutant

Marilena Ignesti et al. Front Physiol. .
No abstract available

Keywords: Drosophila VHL; kidney; microarray analysis; renal carcinoma; sensitized genetic background.

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Figures

Figure 1
Figure 1
MA-plots of differentially expressed genes in dVHL1.1/+ Malpighian tubules. (A) Upregulated genes (average M > 0.5, p < 0.1) are shown in red while downregulated genes (average M<-0.5, p < 0.1) are shown in blue. (B) Genes for which the alteration in gene expression (M > 0.5 or M<-0.5) was recovered in 3 out of 4 slides are shown in green. (C) Genes that satisfy both the cut-off conditions in (A,B) are shown with red triangle.
Figure 2
Figure 2
Validation and classification of differentially expressed genes in dVHL1.1/+ Malpighian tubules. (A) Fold change in transcription levels of dGrip75 and CG31955 genes in the microarray analysis. (B) qRT-PCR analysis of dGrip75 and CG31955 transcript levels in individuals of the reported genotypes. 3 biological replicates of tubules from 5-days old dVHL1.1/+ and wild type females were analyzed. Graphs represent mean ± SD; p = 0.0152 (dGrip75) and p = 0.0153 (CG31955).
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