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. 2019 Mar 27:30:100536.
doi: 10.1016/j.nmni.2019.100536. eCollection 2019 Jul.

Virologic study of acute lower respiratory tract infections in children admitted to the paediatric department of Blida University Hospital, Algeria

F Derrar  1 , K Izri  1 , C Kaddache  2 , R Boukari  2 , D Hannoun  3
Affiliations

Virologic study of acute lower respiratory tract infections in children admitted to the paediatric department of Blida University Hospital, Algeria

F Derrar et al. New Microbes New Infect. .

Abstract
in English, French

Acute lower respiratory tract infections (ALRTI) such as pneumonia and bronchiolitis are major causes of mortality and morbidity in children under 5 years of age. The main microbial agents responsible for ALRTI are either bacterial agents (Streptococcus pneumoniae, Haemophilus influenzae type b, Mycoplasma pneumoniae) or viruses (respiratory syncytial virus (RSV, also known as human orthopneumovirus), Myxovirus influenzae, Myxovirus parainfluenzae, adenovirus) [1]. More recently, other viruses (rhinovirus, metapneumovirus, coronavirus, bocavirus) have been implicated in ALRTI; their identification has been facilitated by new molecular biology techniques such as real-time PCR. To our knowledge, these emerging viruses have never been the subject of epidemiologic studies in our country.

Les infections aiguës des voies respiratoires comptent parmi les principales causes de morbidité et de mortalité chez les enfants. Elles se produisent dans le monde entier et constituent l'un des principaux fardeaux mondiaux des maladies chez les enfants. Le but de cette étude était de déterminer l'étiologie virale des infections respiratoires chez les enfants hospitalisés et âgés de moins de 02 ans.Sur une période d'un an, des aspirations nasales été effectuées chez 117 enfants âgés de 15 jours à 02 ans et hospitalisés pour une maladie respiratoire aiguë. Les échantillons prélevés ont été testés pour la présence de 12 virus respiratoires à l'aide d'une RT-PCR multiplex en temps réel. Des agents pathogènes ont été identifiés chez 97 enfants (82,9 %) et ont été fréquemment observés à l'automne et en hiver. Une co-infection a été observée dans 21,4 % des échantillons. Les pathogènes les plus fréquemment détectés étaient le Virus respiratoire syncytial (VRS, 47,9 %), le rhinovirus humain (hRV, 23,1 %), le métapneumovirus humain (hMPV, 22,2 %). Cette étude fournit des faits pertinents sur la circulation des virus respiratoires en Algérie et sur l'importance de l'utilisation de la PCR multiplexe comme outil intéressant pour la détection des virus. Un diagnostic précoce au moment de l'hospitalisation initiale peut réduire la propagation des virus dans les services de pédiatrie et améliorer la prise en charge.

Keywords: ALRT; Algeria; PCR; RSV; children; hMPv.

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Figures

Fig. 1
Fig. 1
Sample breakdown by epidemiologic week.
Fig. 2
Fig. 2
Child breakdown by number of identified viruses.
Fig. 3
Fig. 3
Virus breakdown by epidemiologic week.
Fig. 4
Fig. 4
Respiratory syncytial virus (human orthopneumovirus)-positive sample breakdown by epidemiologic week.
Fig. 5
Fig. 5
Respiratory syncytial virus (human orthopneumovirus)-positive child breakdown by age.
Fig. 6
Fig. 6
Rhinovirus-positive sample breakdown by epidemiologic week.
Fig. 7
Fig. 7
Rhinovirus-positive child breakdown by age.
Fig. 8
Fig. 8
Human metapneumovirus–positive sample breakdown by epidemiologic week.
Fig. 9
Fig. 9
Human metapneumovirus–positive child breakdown by age.
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