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. 2019 May 8:16:100194.
doi: 10.1016/j.jcte.2019.100194. eCollection 2019 Jun.

Urinary metabolic profiles after vitamin D2 versus vitamin D3 supplementation in prediabetes

Laor Chailurkit  1 , Hataikarn Nimitphong  1 , Sunee Saetung  1 , Boonsong Ongphiphadhanakul  1
Affiliations

Urinary metabolic profiles after vitamin D2 versus vitamin D3 supplementation in prediabetes

Laor Chailurkit et al. J Clin Transl Endocrinol. .

Abstract

Aim: To assess the potential biological differences between vitamin D2 and D3 using urinary metabolite profiles in response to vitamin D3 or D2 supplementation.

Method: Subjects consisted of 29 subjects with impaired fasting glucose and/or impaired glucose tolerance. Subjects were randomized into two groups, vitamin D2 (20,000 IU weekly, n = 14) or vitamin D3 (15,000 IU weekly, n = 15). Urine and serum samples were taken at two different time points for each subject (at baseline and at 12 weeks). Urinary metabolite profiling was performed by liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS). Serum calcium was analyzed on an automated biochemical analyzer and serum intact parathyroid hormone was determined by electrochemiluminescence immunoassay.

Results: At baseline, there was no statistically significant difference in clinical characteristics including age, gender, body mass index, waist circumference and 25-hydroxyvitamin D (25(OH)D) levels between the 2 groups. Weekly administration of 20,000 U D2 for 12 weeks resulted in comparable 25(OH)D concentrations as compared to weekly 15,000 U D3 supplementation (97.8 ± 305 vs. 96.8 ± 3.4 nmol/L, p = 0.84). No difference in serum calcium (2.3 ± 0.03 vs. 2.2 ± 0.03 nmol/L, p = 0.52) or intact parathyroid hormone (5.3 ± 0.3 vs. 4.9 ± 0.5 pmol/L, p = 0.54) at 12 weeks was found. Principle component analysis did not reveal apparent segregation of metabolites according to D2 or D3 supplementation. Moreover, using partial least square regression, no apparent separation between the D2 and the D3 group was found. No important metabolite influencing the separation of the D2 from the D3 group was found using variables importance on projection analysis.

Conclusions: At comparable circulating 25(OH)D concentrations, vitamin D2 or D3 supplementation does not appear to result in different urinary metabolite profiles. Our finding does not support a biological difference between vitamin D2 and D3.

Keywords: Metabolomics; Prediabetes; Vitamin D2; Vitamin D3.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Fig. 1
Fig. 1
Overall study design flowchart. IFG = impaired fasting glucose, IGT = impaired glucose tolerance, DM = diabetes mellitus.
Fig. 2
Fig. 2
Principle component analysis (PCA) of metabolites according to vitamin D2 (circle black color) or D3 (triangle gray color) supplementation at baseline and at 12 weeks in prediabetic subjects.
See this image and copyright information in PMC

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