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. 2019 May 24;5(5):e01769.
doi: 10.1016/j.heliyon.2019.e01769. eCollection 2019 May.

The potential chemo preventive effect of ursolic acid isolated from Paulownia tomentosa, against N-diethylnitrosamine: initiated and promoted hepatocarcinogenesis

Sanaa A Ali  1 , Nabaweya A Ibrahim  2 , Magdy M D Mohammed  2 , Seham El-Hawary  3 , Esraa A Refaat  2
Affiliations

The potential chemo preventive effect of ursolic acid isolated from Paulownia tomentosa, against N-diethylnitrosamine: initiated and promoted hepatocarcinogenesis

Sanaa A Ali et al. Heliyon. .

Abstract

The present study discusses the isolation of ursolic acid from the chloroform extract of Paulownia tomentosa (Thunb) Steud fruits and its cytotoxic effect has been assessed in-vitro was performed in different cells lines (A-549, MCF-7, HepG2) and in-vivo using N-diethylnitrosamine. The obtained results revealed that ursolic acid showed significant cytotoxic activity on MCF-7 and HepG2 cell lines in comparison to Doxorubicin as a reference drug. Moreover, we have assessed the inhibitory effects of Paulownia tomentosa fruit chloroform extract and the isolated ursolic acid on hepatocarcinogenesis was carried out for the first time using N-diethylnitrosamine, where the group treated with ursolic acid given orally after 8 weeks of cancer induction showed the most significant results in comparison to the chloroform extract. The effect of ursolic acid on intoxicated rats caused significant restoration of most of the normal hepatocytes architecture with regular dark nuclei and the group treated with Paulownia tomentosa fruits showed remarkable results with improvement in biochemical analysis.

Keywords: Biochemistry; Natural product chemistry; Pharmaceutical chemistry.

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Figures

Fig. 1
Fig. 1
Chemical structure of ursolic acid.
Fig. 2
Fig. 2
Mass spectra of ursolic acid compound.
Fig. 3
Fig. 3
The hepatoprotective activity of the chloroform fruits extract of Paulownia tomentosa (Thunb.) Steud. & isolated ursolic acid on serum ALT, AST and ALP of rats.
Fig. 4
Fig. 4
The hepatoprotective activity of the chloroform fruits extract of Paulownia tomentosa (Thunb.) Steud. & isolated ursolic acid on GGT, glucose, albumin and total protein of rats.
Fig. 5
Fig. 5
The hepatoprotective activity of the chloroform fruits extract of Paulownia tomentosa (Thunb.) Steud. & isolated ursolic acid on liver weigh, body weight and liver ratio.
Fig. 6
Fig. 6
Liver sections of control animals [H&E] stain (A), (A*) normal hepatocyte cell Masson’ trichome stains. (B) DEN group [H&E] stain. (B*) collagen increase distribution stained with Masson trichome. (C) Revealed the effect of ursolic acid [H&E] stain (C)*the same section stained with. Masson's trichome. (D) H&E stain cirrhotic rats treated with fruits extracts (D*) Masson’ trichome stain showing initial improvement.
Fig. 7
Fig. 7
A quantitative analysis of Fig. 6.
See this image and copyright information in PMC

References

    1. Bansal A.K., Bansal M., Soni G., Bhavnagar D. Protective role of Vitamin E pre-treatment on N-nitrosodiethylamine induced oxidative stress in rat liver. Chem. Biol. Interact. 2005;156:3101–3111. - PubMed
    1. Valan M.F., John de britto A., Venkataraman R. Phytoconstituents with hepatoprotective activity. Int. J. Chem. Sci. 2010;8(3):1421–1432.
    1. Kagawa M., Sano T., Ishibashi N., Hashimoto M., Okuno M., Moriwaki H. An acyclic retinoid, NIK-333, inhibits N-diethylnitrosamine-induced rat hepatocarcinogenesis through suppression of TGF-α expression and cell proliferation. Carcinogenesis. 2004;25(6):979–985. - PubMed
    1. Hamed M.A., Aly H.F., Ali S.A., Metwalley N.S., Hassan S.A., Ahmed S.A. In vitro and in vivo assessment of some functional foods against initiation of hepatocellular carcinoma. J. Basic. Appl. Sci. Res. 2012;2(1):471–483.
    1. Ali S.A., Ahmed S.A., Farrag A.H. Protective and prophylactic role of brassicaceae vegetables extracts on N-nitrosodiethylamine induced initiation of hepatocellular carcinoma in rat liver. Res. Rev.: Journal of Pharmacology and Toxicological Studies. 2015;1:1–19.

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